安徽农业科学2013，Vol.41Issue(4)：1438-1441,4.
TAT PTD-Tachyplesin融合基因的重叠延伸PCR法合成
The Artificial Synthesis of TAT PTD and Tachyplesin Fusion Gene by Overlap Extension PCR
摘要
Abstract
In order to study the coefficient of the TAT PTD (the protein transduction domain in HIV-1 transactivator of transcription protein) to the antibacterial peptide tachyplesin from Tachpleus tridentatus, 6 sequence of oligonucleotides with EcoR land Xba I in the ends of 5'-end of the first and last primers respectively were designed, based on the TAT PTD and tachyplesin mature peptide cDNA template, which was optimized according to the Pichia pastoris preferred codon. A optimized TAT PTD and tachyplesin fusion gene with 219 base pairs was artificially synthesized by overlap extension PCR, which laid a preliminary foundation for the following functional and coefficient studies.关键词
PTD TAT/鲎素/重叠延伸PCRKey words
TAT protein transduction domain/ Tachyplesin/ Overlap extension PCR分类
农业科技引用本文复制引用
邱道寿,刘晓津,王军,苏永全..TAT PTD-Tachyplesin融合基因的重叠延伸PCR法合成[J].安徽农业科学,2013,41(4):1438-1441,4.基金项目
广东省自然科学基金(06025389) （06025389）
福建省博士后项目. （）
