安徽农业科学2013，Vol.41Issue(4)：1459-1461,3.
甘肃枸杞属植物的RAPD分析
Analysis of Wolfberry (Lycium L.) in Gansu by RAPD
陈刚 1刘津 1马志刚 1程亚坤 1王响元1
作者信息
摘要
Abstract
[Objective] To study the genetic relationship among species in Lycium L. of Gansu Province. [ Method] The genomic DNA was extracted from Lycium L. in Gansu Province and its polymorphism and genetic relationship were analyzed by RAPD and UPGMA cluster method. [Result] 28 primers were screened from 80 random primers of 10 bases for RAPD analysis of 6 samples. By using 28 primers, a total of 171 bands were obtained and 118(69.0% ) showed polymorphism. The genetic similarity coefficients were between 0. 39 and 0.70 and the average value was 0. 59. [Conclusion] Cluster analysis indicated that the closer relationship was between L. chinense Mill, and L. dasyslemum Pojark. , Lycium barbarum L. , L, chinese Mill. var. potaninii(Pojark. )A. M. Lu and L. trunlcatum Y. C. Wang were clustered together. L. ru-thenicum Murr. is highly divergent from other species.关键词
枸杞/RAPD/DNA指纹图谱/聚类分析Key words
Lycium. L. / RAPD/ DNA fingerprint/ Cluster analysis分类
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陈刚,刘津,马志刚,程亚坤,王响元..甘肃枸杞属植物的RAPD分析[J].安徽农业科学,2013,41(4):1459-1461,3.
