安徽农业科学2013，Vol.41Issue(4)：1462-1463,1503,3.
‘幻想’矮牵牛遗传转化受体再生体系建立
Establishment of Regeneration System for Genetic Transformation of Petunia hybrida ' Fantasy'
摘要
Abstract
[ Objective ] The paper was to establish efficient regeneration system for genetic transformation of Petunia hybrida ' Fantasy'. [ Method ] The effects of dark incubation time, auxin types and concentrations of sprouting medium and rooting medium, screening antibiotics and antibacterial antibiotics on shoot regeneration and rooting were studied by using young leaves of P. hybrida ' Fantasy' as explants. [ Result] The best dark incubation time for the induction of leaf differentiation was 2 days; the best medium for sprouting was MS + 6-BA 2. 0 mg/L + NAA 0. 2 mg/L; the best rooting medium was MS + IBA 0. 1 mg/L; the appropriate kanamycin screening pressure in the sprouting and rooting stage was 15 mg/L; the suitable concentration of antibacterial antibiotics cefotaxime was 300 mg/L. [Conclusion] The study laid the foundation for the late molecular and breeding research of P. hybrida ' Fantasy'.关键词
‘幻想’矮牵牛/遗传转化/再生体系/暗培养时间/生长素/抗生素Key words
Petunia hybrida ' Fantasy' / Genetic transformation/ Regeneration system/ Dark incubation time/ Auxin/ Antibiotics分类
农业科技引用本文复制引用
马方芳,岳远征,黄雪,孙健,胡惠蓉..‘幻想’矮牵牛遗传转化受体再生体系建立[J].安徽农业科学,2013,41(4):1462-1463,1503,3.基金项目
国家自然科学基金资助项目(30972020). （30972020）
