北京中医药大学学报2013，Vol.36Issue(2)：96-99,后插1,5.DOI:10.3969/j.issn.1006-2157.2013.02.007
黄芪和葛根素对2型糖尿病KKAy小鼠的肾脏保护作用及纤黏连蛋白表达的影响
Protective effects of Huangqi Injection and Puerarin Injection on kidney and their influences on expression of fibronectin in KKAy mice with type 2 diabetes
摘要
Abstract
Objective To observe the protective effects of Huangqi Injection and Puerarin Injection on kidney and their influences on expression of fibronectin (FN) in KKAy mice with type 2 diabetes based on the changes of kidney function and renal morphology. Methods Male KKAy mice were divided into model group and treatment group (treated with Huangqi Injection combining Puerarin Injection) , and male same-aged C57BL/6J mice were taken as normal group. The levels of plasma glucose, LDL-C,HDL-C and serum urea and uric acid (UA) were regularly detected. The pathological changes of renal tissue were observed after HE and Masson staining, and FN expression was observed by using immunohistochemistry technique. Results Started from 20 weeks old, the levels of plasma glucose, blood fat and serum urea and (UA) increased significantly in model group than those in normal group, and improved in treatment group compared with model group. HE and Masson staining showed glomerular sclerosis, vacuolar degeneration in renal tubular epithelial cells and more interstitial collagen in model group, and these pathological changes were significantly relieved in treatment group. The results of immunohistochemistry technique showed that FN expression increased significantly in model group than that in normal group, and decreased in treatment group than that in model group. Conclusion Huangqi Injection combining Puerarin Injection can improve kidney function, alleviate renal pathological changes, reduce FN expression and protect kidney in KKAy mice with with type 2 diabetes.关键词
KKAy小鼠/糖尿病肾病/黄芪注射液/葛根素注射液/纤黏连蛋白Key words
KKAy mice/ diabetic nephropathy/ Huangqi Injection/ Puerarin Injection/ fibronectin分类
医药卫生引用本文复制引用
李姝玉,柴欣楼,吴莹,苏玮莲,贾德贤,郝钰,王谦..黄芪和葛根素对2型糖尿病KKAy小鼠的肾脏保护作用及纤黏连蛋白表达的影响[J].北京中医药大学学报,2013,36(2):96-99,后插1,5.基金项目
国家自然科学基金资助项目(No.30672756,81072926),北京中医药大学创新团队资助项目(No.2011-CXD-04) （No.30672756,81072926）
