北京中医药大学学报2013，Vol.36Issue(2)：100-103,4.DOI:10.3969/j.issn.1006-2157.2013.02.008
应用基因芯片技术检测金雀异黄素对乳腺癌T47D细胞雌激素受体相关信号通路基因表达的影响
Influence of genistein on gene expression of estrogen receptor-related signal pathway detected with cDNA microarray technique in breast cancerT47D cells
摘要
Abstract
Objective To observe the influences of genistein on the gene expression of estrogen (E2) -dependent breast cancer T47D cells and estrogen receptor-dependent signal transduction pathway by applying DNA oligo microarray technique. Methods T47D cells were respectively processed with 0.001μmol/L E2 and 10 μmol/L genistein for 48 horns. The total mRNA was extracted after collecting cells, labeled through reverse transcription reaction, and then hybridized with Oligo GEArray? chips. Differential expression genes were collected from the chips after computer scanning. Results The expressions of Cyclin E1, Cyclin E2 and Cyclin A2 were all up-regulated significantly. The expressions of ERa, and E2-induced PGR, TFF1 (PS2), BCL2, ERBB2, C3, CCND1, TEF3, IGFBP2, IGFBP5 and GATA3 were also up-regulated in varying degrees. The results of real-time fluorescence quantitative polymerase chain reaction (RT-PCR) showed that genistein induced the expression of pS2 mRNA. Conclusion Genistein can activate the expression of ERa receptor in T47D cells, regulate cyclin dependent kinase(CDK) activity to accelerate S phase, improve the proliferation of T47D cells, and play a role of phytoestrogen through up-regulating E2-induced genes.关键词
植物雌激素/金雀异黄素/基因芯片/雌激素受体Key words
phytoestrogen/ genistein/ DNA microarray/ estrogen receptor分类
医药卫生引用本文复制引用
沈丽霞,牛建昭,王继峰..应用基因芯片技术检测金雀异黄素对乳腺癌T47D细胞雌激素受体相关信号通路基因表达的影响[J].北京中医药大学学报,2013,36(2):100-103,4.基金项目
国家自然科学基金资助项目(No.307725849),河北省自然科学基金资助项目(No.H2012405016) （No.307725849）
