北京中医药大学学报2013，Vol.36Issue(2)：113-116,封3,5.DOI:10.3969/j.issn.1006-2157.2013.02.011
茵陈蒿汤水提物和乙醇提物对D-氨基半乳糖胺诱导大鼠急性肝损伤保护作用的比较研究
Protective effects of aqueous extract and ethanol extract of Yinchenhao Tang on liver in rats with acute liver injury induced by D-galactosamine
摘要
Abstract
Objective To compare the difference in the protective effects on liver in rats with acute liver injury induced by D-galactosamine ( D-GalN) between the aqueous extract and ethanol extract of Yinchenhao Tang. Methods Health male Wistar rats were randomly divided into normal group, model group, groups of high-dose and low-dose aqueous extract of Yinchenhao Tang ( high-dose and low-dose aqueous extract groups) , and groups of high-dose and low-dose ethanol extract of Yinchenhao Tang ( high-dose and low-dose ethanol extract groups). Except of normal group, other groups were preventively given medicinal for 7 days, and then given intraperitoneal injection of D-GalN one for copying the model of acute liver injury. The changes of serum alanine aminotransferase (ALT) , aspartate aminotransferase (AST) , triglyceride (TG) , total bilirubin (T-BiL) , superoxide dismutase (SOD) and malondialdehyde (MDA) were detected. At the same time the paraffin sections and frozen sections of liver tissue were prepared and stained with HE, oil red-O, SDH and G-6-P staining for light microscope observation and photographs. Results Multi-factor analysis of variance showed that the activity of decreasing TG and T-BiL of aqueous extract was higher than that of ethanol extract ( P < 0. 05 ) , while inhibitory effect of ethanol extract on MDA was higher than that of aqueous extract. The pathological results indicated that aqueous extract had better liver protective effect than ethanol extract. Conclusion The aqueous extract technique and ethanol extraction technique of Yinchenhao Tang can obtain different effective components/ fractions or groups for playing different pharmacodynamic effects. For reducing blood fat and bilirubin, traditional decocting technique is the first selection, and for antioxidant and anti-inflammatory activities, the ethanol extract technique will be better.关键词
D-氨基半乳糖胺/急性肝损伤/茵陈蒿汤/水提取物/乙醇提取物/大鼠Key words
D-galactosamine/ acute liver injury/ Yinchenhao Tang/ aqueous extract/ ethanol extract/ rats分类
医药卫生引用本文复制引用
张慧芹,蔡华丹,杨美娟,郝钰,李军祥,李健..茵陈蒿汤水提物和乙醇提物对D-氨基半乳糖胺诱导大鼠急性肝损伤保护作用的比较研究[J].北京中医药大学学报,2013,36(2):113-116,封3,5.基金项目
北京中医药大学自主选题基金资助项目(No.JYBZZ-JS004),北京中医药大学2011年创新团队资助项目 （No.JYBZZ-JS004）
