北京中医药大学学报2013，Vol.36Issue(2)：117-120,4.DOI:10.3969/j.issn.1006-2157.2013.02.012
不同产地铁皮石斛主要化学成分及指纹图谱研究
Main chemical compositions and fingerprints of Tiepishihu (Dendrobium candidum) produced from different areas
摘要
Abstract
Objective To compare the quality of Tiepishihu (Dendrobium candidum) produced from different areas in Yunnan Province, and provide evidence for selecting its optimal producing area. Methods The hot-dip method, ultraviolet spectrophotometry and HPLC were applied to determine the content of alcohol extracts, polysaccharide and mannose in Tiepishihu and at the same time, HPLC gradient elution was used to establish fingerprint system, which then was given cluster analysis. Results Tiepishihu produced from different areas in Yunnan Province had difference in the content of main chemical compositions. The content of alcohol extracts (13.65% ) and polysaccharide (35.87% ) was the highest in Tiepishihu produced from Menghai. The content of mannose was the highest (24.%% ) in Tiepishihu produced from Dehong and was secondary highest (24.01%) in Tiepishihu produced from Menghai. The content of effective compositions was the lowest in Tiepishihu produced from Honghe. The similarity of HPLC fingerprints of Tiepishihu produced from different areas was higher (between 0.883 and 0.987), while the similarity was the lowest (0.883) between Tiepishihu produced from Menghai and control fingerprint, and that among other Tiepishihu products were all higher than 0.950. The cluster analysis on the total area of HPLC fingerprint common peaks of Tiepishihu produced from 6 areas showed that these 6 areas could be divided into two categories, one included Menghai and Wenshan, and other one included Simao, Dehong, Honghe and Yuxi, Conclusion Tiepishihu produced from different areas in Yunnan Province has difference in quality, and that produced from Menghai is the best.关键词
铁皮石斛/不同产地/化学成分/指纹图谱/聚类分析Key words
Tiepishihu ( Dendrobium candidum) / different producing areas/ chemical compositions/ fingerprints/ cluster analysis分类
医药卫生引用本文复制引用
刘文杰,孙志蓉,杜远,张世定,郭培俊,彭贵湖,陈龙..不同产地铁皮石斛主要化学成分及指纹图谱研究[J].北京中医药大学学报,2013,36(2):117-120,4.基金项目
"十二五"国家科技支撑计划资助项目(No.2011BAI13B02-5) （No.2011BAI13B02-5）
