北京中医药大学学报2013，Vol.36Issue(2)：138-141,4.DOI:10.3969/j.issn.1006-2157.2013.02.017
慢性阻塞性肺疾病稳定期肺气虚证及其中药干预的尿液代谢组学研究
Urine metabolomics study on lung qi deficiency syndrome of stable-phase chronic obstructive pulmonary disease and intervention of Chinese medicinal
摘要
Abstract
Objective To study the characteristics of urine metabolomics of lung qi deficiency syndrome of stable-phase chronic obstructive pulmonary disease (COPD) and intervention of Chinese medicinal, and explore possible biomarkers. Methods The patients (n =40) with lung qi deficiency syndrome of stable-phase COPD (lung qi deficiency group) were intervened with Liuwei Buqi Capsules, and a control group (n = 37 ) was set up. The two groups were given the examination of lung function before and after the intervention. The urine metabolites were detected by using HPLC-MS/MS, and data was statistically analyzed by applying principle component analysis (PCA) and partial least squares (PLS). The original spectrum and data of urine metabolomics were compared between two groups. Results Compared with control group, lung function decreased significantly in lung qi deficiency group before treatment (P<0.05), and still lower after treatment for 30 days. The metabolic spectrum was significantly different in lung qi deficiency group compared with control group before treatment, but showed regressive trend to control group after treatment. There were 13 possible biomarkers of lung qi deficiency syndrome of COPD found. Conclusion The changes of metabolic spectrum and potential disease biomarkers can be found in patients with lung qi deficiency syndrome of stable-phase COPD The intervention of Chinese medicinal will bring the positive changes in metabolic spectrum.关键词
代谢组学/慢性阻塞性肺疾病/稳定期/肺气虚证/六味补气胶囊/尿液Key words
metabolomics/ chronic obstructive pulmonary disease/ stable phase/ lung qi deficiency/ Liuwei Buqi Capsules/ urine分类
医药卫生引用本文复制引用
李泽庚,刘志刚,徐彬,彭波,章天寿,季红燕,童佳兵,杨程,杨志来..慢性阻塞性肺疾病稳定期肺气虚证及其中药干预的尿液代谢组学研究[J].北京中医药大学学报,2013,36(2):138-141,4.基金项目
国家自然科学基金资助项目(No.81270781),安徽中医学院青年科学研究基金资助项目(No.2010qn006) （No.81270781）
