重庆医学2013，Vol.42Issue(9)：961-963,3.DOI:10.3969/j.issn.1671-8348.2013.09.001
脊髓Toll样受体4在慢性瘙痒中的作用研究
Role of spinal Toll-like receptor 4 in chronic pruritus*
摘要
Abstract
Objective To investigate the role of spinal Toll-like receptor 4(TLR4) in chronic pruritix induced by repeated administration of oxazolone. Methods 152 male kunming mice were selected and divided into 3 groups with 40 mice in each group:the contol group(C) .acetone group( A) and oxazolone group(O) ,and each group was redivided into 5 sub-groups including before first stimulation,on 0,4,9 11 d stimulation sub-groups. Mice in each group were treated with nothing,acetone or oxazolone. The scratching behaviors were observed for 30 min after every stimulation, then the cervical enlargement of spinal cord of mice was used to test the expression of TLR4. To investigate the effect of intraperitonieal injection of EGCG(40 mg/kg) ,mice were randomly divided into four groups: acetone group( A) ,acctonc+EGCG group( A + E) , oxazolone group(O) and oxazolone + EGCG group(O+E). EGCG was intraperitoneally injected from 8 h after stimulation on 9 d,onc time/8 h.for 6 times to the beginning of the last stimulation on 1 1 d. The TLR4 expression from mice was detected with Western blot assaying 30 min after the last stimulation on 11 d. Results From the first stimulation,the scratching behaviors at various time points in the group O were significantly more than those in the group C and A(P<0. 05) , and the spinal TLR4 expression was positively correlated with the scratching behaviors(r = 0. 947). Conclusion Intraperitoneal injection of EGCG could reduce the spinal TLR4 expression and the scratching behaviors. Spinal TLR4 mediates the chronic pruritis induced by repeated administration of oxazolone.关键词
噁唑酮/瘙痒/Toll样受体4Key words
oxazolone/pruritis/Toll-like receptor 4引用本文复制引用
孔敏,周芳,党秀静,武静茹..脊髓Toll样受体4在慢性瘙痒中的作用研究[J].重庆医学,2013,42(9):961-963,3.基金项目
国家自然科学基金青年基金项目(30900417) （30900417）
徐州市科技计划项目(XM12B017). （XM12B017）
