重庆医学2013，Vol.42Issue(9)：964-967,4.DOI:10.3969/j.issn.1671-8348.2013.09.002
糖尿病对大鼠肝纤维化组织TIMP-1/MMP-1表达的影响
Effect of diabetes on expression of TIMP-1/MMP-1 in rat hepatic fibrosis tissue
摘要
Abstract
Objective To investigate the effect of diabetes on the expression of metalloproteinase-1 (TIMP-1)/ matrix metallo-proteinasc-1 (MMP-1) in hepatic fibrosis rats. Methods 40 healthy male SD rats were randomly divided into four groups: normal control group,diabetes group,liver fibrosis group and diabetes liver fibrosis group(doublc model group) , 10 rats in each group. The diabetes group and the double model group were intraperitoneally injected with STZ to establish diabetes model. The liver fibrosis group and the double model group were subcutaneously injected with 40% carbon tetrachloride(CCl4) peanut oil solution to establish the liver fibrosis model. At 48 h after final injection of CC14 , the blood sample was collected from heart. Serum alanine amin-otransferase( ALT) , glutamic-oxaloacetic transaminase(AST) , albumin( ALB) and albnmin/glonmin( A/G) were detected by the automatic biochemical detector. Hepatic fibrosis indexes,such as hyaluronic acid(HA) ,layer-attached proteins(LN) ,procollagen type-Ⅲ (PⅢNP) , Ⅳ type collagen(C-Ⅳ ) ,were detected by the chemiluminescent method. Live tissue sections were stained with HE and VG to observe degeneration necrosis and fibrosis levels. The levels of mRNA expression of α-SMA, MMP-1 and TIMP-1 were detected by the real-time PCR. Results Compared with the control group and the diabetes group, scrum ALT, AST, HA,LN,PⅢ NP and C-Ⅳ levels in the liver fibrosis group and the double model group were increased, ALB and A/G were decreased,and α-SMA, TIMP-l/MMP-1 and TIMP-1 gene expression was increased, the differences were statistically significant (P< 0. 05). Compared with the liver fibrosis group, the serum concentrations of ALT, AST, HA and C-Ⅳ in the double model group were increased, α-SMA,TIMP-1 and TIMP-1/MMP-1 gene expression was increased and the scores of liver fibrosis were increased, the differences were statistically significant(P<0. 05). Conclusion Diabetes can promote and increase the occurrence and development of hepatic fibrosis,TIMP-l/MMP-1 imbalance intensifies the extracellular cell matrix(ECM) deposits.关键词
糖尿病/肝纤维化/α-平滑肌肌动蛋白/金属基质蛋白酶-1/金属蛋白酶组织抑制剂-1Key words
diabetes mellitus/hepatic fibrosis/α-SMA/MMP-1/TIMP-1引用本文复制引用
陈枫,杨爱萍,李燕,史小玲,唐小平,唐莉,王晓燕,陈庄..糖尿病对大鼠肝纤维化组织TIMP-1/MMP-1表达的影响[J].重庆医学,2013,42(9):964-967,4.基金项目
四川省重点学科建设项目(SCD0241). （SCD0241）
