重庆医学2013，Vol.42Issue(9)：1002-1004,3.DOI:10.3969/j.issn.1671-8348.2013.09.016
吡咯烷二硫代氨基甲酸盐对喉癌Hep-2细胞增殖和凋亡的影响
Effects of PDTC to proliferation and apoptosis of Hep-2 cells
曹雪秋1
作者信息
- 1. 湖北民族学院附属民大医院耳鼻喉头颈外科,湖北恩施,445000
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摘要
Abstract
Objective To observe the effects of ammonium pyrrolidinedithiocarbamat(PDTC) to apoptosis and proliferation of Hep-2 cells. Methods Hep-2 cells were cultured in vitro. The control group and PDTC treatment groups were established according to the experimental requirements. The methyl thiazolyl tetrazolium(MTT) assay was used to measure the growth inhibition rate of Hep-2 cells. The flow cytometry assay was used to determine the apoptosis in Hep-2 cells. Bcl-xL and XIAP mRNA levels were measured by real time PCR in untreated Hep-2 cells and cells treated for varying amounts of PDTC. Western-blot analysis was used to determine the protein levels of p-IκB, Bcl-xL and XIAP in Hcp-2 cells. Results The 50 μmol/L and 100 μmol/L PDTC groups inhibited the growth of Hep-2 cells, difference compared with the control group showing statistical significance. The greater the PDTC concentration was,the more significant the inhibition effect was(P<0. 05). After 24 h treating by PDTC,the apoptosis rate of Hcp-2 cells in the 50 μmol/L and 100 μmol/L PDTC groups wcrc(25. 1 ± 2. 18)% and(35. 4 ± 2. 62)% respectively, difference compared with the control group[(3. 12±1. 07)%] showing statistical significance. PDTC could lead to the growth inhibition of Hcp-2 cells and the increase of apoptosis with the dose-effect relationship. Conclusion PDTC inhibits the proliferation of Hep-2 cells and promotes their apoptosis by blocking the NF-ΚB activation of Hep-2 cells and reducing the expression of Bcl-xL and XIAP.关键词
喉肿瘤/吡咯烷二硫代氨基甲酸盐/Hep-2细胞/增殖/凋亡Key words
laryngocarcinoma/PDTC/ Hep-2 cells/proliferation/apoptosis引用本文复制引用
曹雪秋..吡咯烷二硫代氨基甲酸盐对喉癌Hep-2细胞增殖和凋亡的影响[J].重庆医学,2013,42(9):1002-1004,3.
