福州大学学报（自然科学版）2013，Vol.41Issue(1)：115-120,6.DOI:10.7631/issn.1000-2243.2013.01.0115
一株产高温纤维素酶曲霉菌的发酵条件及培养基优化
Optimization of medium composition and fermentation conditions for the production of thermophilic cellulase by Aspergillus sp·
摘要
Abstract
An Aspergillus sp. strain F4 capable of producing thermophilic cellulases was previously selected in the laboratory. The studies presented here described the optimization of medium components and process parameters for cellulase production by the strain using liquid fermentation. The effect of carbon sources, nitrogen sources, salts and initial culture pH on enzyme production was evaluated, and the best fermentation medium was found to be: 40 g · L-1 rice straw powder, 3. 5 g · L-1 (NH4)2SO4, 2g·L-1 NaCl, 2g· L-1 KH2PO4, 0.5 g· L-1 MgSO4 -7H2O, 0.2% glycerine, pH 5.5, which resulted in a 16.7 -fold increase of CMCase yield. The inoculum size of 4% and culture volume of 80 mL/250 mL flask were optimum for cellulase production by strain F4. When incubated at 37 °C with the shaking speed of 150 r · min-1. highest CMCase activity of 12.26 U · mL-1 (with another 2 - fold increase) was seen after a period of 8 d.关键词
纤维素酶/培养基/发酵条件/优化Key words
cellulase/ medium composition/ fermentation conditions/ optimization分类
化学化工引用本文复制引用
杨捷,严芬,叶秀云,李仁宽,刘志坚,吕文静,林娟..一株产高温纤维素酶曲霉菌的发酵条件及培养基优化[J].福州大学学报（自然科学版）,2013,41(1):115-120,6.基金项目
福建省科技厅科研资助项目(2010J05073) （2010J05073）
福州大学人才基金资助项目(XRC-1032,XRC-0946) （XRC-1032,XRC-0946）
福州大学科技发展基金资助项目(2010-XQ-19) （2010-XQ-19）
福建省发改委生物技术开发专项资助项目(闽发改高技[2011]1598号) （闽发改高技[2011]1598号）
