中医学报2013，Vol.28Issue(3)：390-391,400,3.
紫外分光光度法测定葎草中总黄酮含量
Ultraviolet Spectrophotometry Information for Total Content of Flavonoids in the Grass
摘要
Abstract
Objective: To investigate the Humulus scandens content determination of total flavone. Methods: Using rutin as control sample , using ultrasonic extraction method to extract total flavonoids, with sodium nitrite-aluminum chloride-sodium hydroxide colorimetry, optimum chromogenic conditions: sodium nitrite concentration 5% , aluminum chloride concentration10% , sodium hydroxide concentration 4%. Through ultraviolet visible spectrophotometric determination of total flavone content. Results:The detection wavelength was 510 nm,rutin at a concentration of 0.024 1 -0.064 0 g·L-1 range is a good linear relationship,the regression equation:y =0. 081 7x - 0. 001 8(R2 = 0. 999 5 ), 1H ( RSD = 0. 364% ) basic stability, good reproducibility ( RSD = 0. 600% ) , sample recovery rate was 99, 46%(RSD = 1. 685% ). Conclusion: UV visible spectrophotometric determination of total flavone content in Humulus scandens, method is simple,fast,accurate,and reliable.关键词
葎草/总黄酮/超声提取法/紫外可见分光光度法Key words
humulus/ total flavone/ ultrasonic extraction/ ultraviolet visible spectrophotometry分类
医药卫生引用本文复制引用
赵若晗,石秀梅..紫外分光光度法测定葎草中总黄酮含量[J].中医学报,2013,28(3):390-391,400,3.基金项目
牡丹江医学院科学技术研究项目(编号:2010-31) （编号:2010-31）
