实用临床医药杂志2013，Vol.17Issue(1)：27-29,33,4.DOI:10.7619/jcmp.201301008
建立幽门螺杆菌感染BALB/c小鼠动物模型
The establishment of BALB/c mouse model with Helicobacter pylori infection
摘要
Abstract
Objective In common breeding condition the animal model is established by the Yunnan H. pylori strain on the BABL/c mice. Methods Taking 0.2 mol/L NaHCO3 orally, after 15 minutes we pour the mice H. Pylori 3-21 strain five times continually, and finished in a week. After 4 weeks of infection, all the mice were killed, and three pieces of the gastric mucosa were obtained from pyloric potion, one was used for rapid urease testing, one for histopathological detections, and the rest one was used for PCR-RFLP is used to confirm if the infected strain is the pre-strain which is fed into the mice' mouths. Results After 4 weeks of in fection, the killed mice were confirmed be infected H. Pylori by the histopathological detections, rapid urease testing and culture of H. pylori. The normal control group was negative. Conclusions The animal model, BABL/c mice of infection is established by Yunnan H. pylori strains carrying cagA genes and vacA gene successfully.关键词
幽门螺杆菌/动物模型/感染Key words
Helicobacter Pylori/ the animal model分类
医药卫生引用本文复制引用
罗萍,周曾芬..建立幽门螺杆菌感染BALB/c小鼠动物模型[J].实用临床医药杂志,2013,17(1):27-29,33,4.
