口腔颌面外科杂志2013，Vol.23Issue(1)：18-22,5.DOI:10.3969/j.issn.1005-4979.2013.01.004
VEGF-ASODN转染SACC-2细胞对其VEGF表达及生长的影响
Expression and Significance of VEGF in Salivary Adenoid Cystic Carcinoma Cells in Association with VEGF-ASODN Transfection
摘要
Abstract
Objective: The study was designed to observe whether VEGF-antisense oligonucleotide (VEGF-ASODN) transfection to SACC-2 cells are associated with its expression of vascular endothelial growth factor (VEGF) and its' pathological significances.Methods: The VEGF-ASODN was synthesized artificially with phosphorothioic acid.After transfecting with VEGF-ASODN into SACC-2, the quantity of VEGF mRNA and protein was detected by in situ hybridization and immunohistochemistry.The quantity of VEGF protein in supernatant was detected by ELISA.Flow Cytometry and MTT assay were used to detect cell activity and cellular apoptosis.SPSS 13.0 software was used for statistical analysis.Results: VEGF mRNA protein levels within the cells and in culture medium both decreased significantly after transfected by VEGF-ASODN (P<0.01 ,P<0.05).The ratio of SACC-2 cell apoptosis was increased.The activities of cells were also decreased after transfected by VEGF-ASODN (P<0.01).Conclusion: Transfection with VEGF-ASODN in SACC-2 can markedly inhibit expression of VEGF.It can enhance cellular apoptosis and suppress pathological growth of these cells.关键词
血管内皮细胞生长因子/反义寡核苷酸/转染/涎腺腺样囊性癌ACC-2细胞Key words
vascular endothelial growth factor/ antisense oligonucleotide/ transfection/ salivary adenoid cystic carcinoma cell ACC-2分类
医药卫生引用本文复制引用
李晓光,李腾宇,王旭霞,王延秀,肖长杰,徐晓,李俊福..VEGF-ASODN转染SACC-2细胞对其VEGF表达及生长的影响[J].口腔颌面外科杂志,2013,23(1):18-22,5.基金项目
山东省科学技术发展计划资助项目(2011GGH21822) （2011GGH21822）
