老年医学与保健2013，Vol.19Issue(1)：37-40,4.DOI:10.3969/j.issn.1008-8296.2013-01-12
鞭毛蛋白致肺微血管内皮细胞炎症诱发共培养肺泡上皮细胞炎症的级联放大效应与信号传导通路
Inflammation cascade effect and signaling transduction pathway of alveolar epithelial cells co-cultured with pulmonary microvascular endothelial cells treated with flagellin
摘要
Abstract
Objective To explore inflammation cascade effects of flagellin infection and signaling transduction pathway. Methods A Transwell coculture system of human pulmonary microvascular endothelial cell (HPMECs) and A549 cells was constructed. HPMECs, which was put into the lower chamber and cultured for 2 hours, were co-cultured with A549 cells put in the upper chamber for 15 days. 3 groups were set as below: HPMECs were co-cultured with A549 cells (control group), HPMECs infected with flagellin (a final concentration of 2 μg/mL) were co-cultured with A549 cells (experimental group Ⅰ) and flagellin-infected HPMECs treated with DAPT (inhibitor of notch signaling, a final concentration of 10 μmol/L) were co-cultured with A549 cells (experimental group Ⅱ ). The proteins of TNF-α and Notch 1 in supernatant of HPMECs and A549 cells in all cultured groups were evaluated by ELISA assay, respectively. Results Compared with the control group, the levels of proteins of TNF-α and Notchl in supernatant of HPMECs [(11.45 ±1.59) pg/mL vs (6.13±0.86) pg/mL, (P<0.01), (7.03 ± 1.06) pg/mL vs (5.39±0.76) pg/mL, (P<0.05)] and supernatant levels of TNF-α in A549 cells [(9.93±1.46)pg/mLvs (5.89±0.83)pg/mL, (P<0.01)] in all cultured groups were significantly increased in the experimental group Ⅰ. These results indicated that flagellin caused HPMECs inflammation, which was enlarged and transducted to the co-cultured A549 cells, whereas this phenomenon was inhibited by using DAPT, indicating that A549 cells inflammation induced by flagellin-infected HPMECs was transducted through Notch signaling pathway. Conclusion Flagellin infection can cause inflammation of pulmonary microvascular endothelial cells, which may be enlarged and transducted to alveolar epithelial cells through the Notch signaling pathway.关键词
鞭毛蛋白/微血管内皮细胞/肺泡上皮细胞/A549细胞/炎症/Notch信号通路/共培养Key words
Flagellin/ Pulmonary microvascular endothelial cells/ Alveolar epithelial cell/ A549 cells/ Inflammation/ Notch signaling pathway/ Coculture分类
医药卫生引用本文复制引用
周长喜,夏世金,胡明冬,杨昱,孙涛,钱桂生..鞭毛蛋白致肺微血管内皮细胞炎症诱发共培养肺泡上皮细胞炎症的级联放大效应与信号传导通路[J].老年医学与保健,2013,19(1):37-40,4.基金项目
国家自然科学基金(81270115) （81270115）
