生命科学研究2013，Vol.17Issue(1)：5-10,6.
电穿孔转染重组杆状病毒到昆虫细胞的研究
The Research of Electroporation Transfect Restructured Baculovirus Genome into Insects Cells
摘要
Abstract
The basic ion liposome, such as Cellfectin, is usually used to transfect cell at present. The shortage of this method is expensive and instable. While the literature report that transfect exogenous gene into insects cells by electroporation is very rare. Construct the restructured baculovirus genome GFP/BAC and transfect into the insects cells Sf9 by electroporation and liposome Cellfectin, then compare the transfection efficiency of these two methods. The result show that the transfection efficiency of the two methods respectively is 86.14% and 86.53%, and the filial generation restructured baculovirus continue transfect Sf9 cell and the fluorescence intensity of these two methods was not obviously distinct. The transfection efficiency of electroporation is compared with liposome Cellfectin, while the advantages of electroporation is simple operation, short cycle and low cost.关键词
电穿孔/转染效率/重组杆状病毒/昆虫细胞Key words
electroporation/ transfection efficiency/ restructured baculovirus/ insects cells分类
生物科学引用本文复制引用
陈卫东,杨靖佳,井申荣,曾韦锟,黄芬..电穿孔转染重组杆状病毒到昆虫细胞的研究[J].生命科学研究,2013,17(1):5-10,6.基金项目
国家自然科学基金资助项目(30872398、31160193) （30872398、31160193）
云南省应用基础研究项目(2007C032M) （2007C032M）
