生命科学研究2013，Vol.17Issue(1)：24-31,8.
红梨PybHLH基因过表达提高烟草NaCl抗性的研究
Overexpression of PybHLH Gene in Tabacco Enhanced its Resistance to NaCl Stress
摘要
Abstract
In order to study the effect of overexpression of PybHLH transcription factor in tobacco on its resistance to NaCl, PybHLH gene was isolated from red peel of Yunan Red Pear. PybHLH protein localized in the nucleus. Both the transgenic tobacco and the wild type were treated with NaCl. The effect of NaCl stress to their physiological and biochemical characteristics and expression profilings of the related enzymes genes were analyzed. The results showed that there was some NaCl resistance in PybHLH transgenic tabacco. On one hand, with the increase of NaCl treatment time, total soluble protein, total soluble sugar and proline contents increased, while H2O2 decreased; on the other hand, proline biosynthesis related gene P5CS, antioxidant related genes MnSOD, CuZnSOD and POD, stress related genes HSP and HSP cheperon, ABA signal pathway related gene NAC were all upregulated. These indicated that overexpression of PybHLH in tabacco enhanced its NaCl tolerance, which will provide foundations for further study on the mechanism of plant NaCl resistance and development of new cultivars with salt-resistance.关键词
PybHLH/转基因烟草/NaCl胁迫/生理特性/基因过表达Key words
PybHLH/ transgenic tobacco/ NaCl stress/ physiological characteristics/ gene overexpression分类
生物科学引用本文复制引用
崔道雷,张晓东,徐慧妮,陈丽梅,舒群,李昆志..红梨PybHLH基因过表达提高烟草NaCl抗性的研究[J].生命科学研究,2013,17(1):24-31,8.基金项目
国家自然科学基金资助项目(31060258) （31060258）
云南省中青年学术技术后备人才培养基金资助项目(2006PY01-10) （2006PY01-10）
