水生生物学报2013，Vol.37Issue(1)：62-69,8.DOI:10.7541/2013.62
异育银鲫体内盐酸双氟沙星血浆蛋白结合率的变化与其药代动力学研究
RESEARCHES OF PLASMA PROTEIN BINDING RATE OF DIFLOXACIN AND ITS PHARMACOKINETIC IN CARASSAIS AURATUS GIBEBIO
摘要
Abstract
To investigate the relationship between the diversities of plasma protein binding rate of Difloxacin (DIF) and its pharmacokinetic in Carassais auratus gibebio, ultrafiIteration and high performance liquid chromatographic (HPLC) methods were applied to determine the plasma protein binding rate and pharmacokinetics of DIF in both infected by Aerominas hydrophila bacteria (infected group) and healthy Carassais auratus gibebio (healthy group), and the relationships between the diversities of plasma protein binding rate of DIF and its pharmacokinetic were analyzed in this paper. The results showed that the plasma protein binding rates of the drug in infected group was significantly higher than that of the healthy group at the corresponding time points. The plasma protein binding rates had logarithmic relationships with the total drug concentration and their equations were y=-9.011n x+74.34(Infected group)and y=-4.811n x+65.15(Healthy group), while the equations between the plasma protein binding rates and free concentration of the drug were y = -6.361n x + 64.91 (Infected group) and y = -4.361n x+ 60.63(Healthy group) in these two groups. Meanwhile the concentration time curve of the two groups were all in accordance with the two-compartment open model. The absorptions and eliminations of DIF in infection group were slower while its apparent distribution volumes and the area under the concentration-time curves were greater than that of the healthy group. The results indicate that the values of the plasma protein binding rates of DIF could be increased by Aeromonas hydrophila in crucian carp, and this may be one of the reasons that leads to limit distribution of drugs, elimination of slowing down and a long stay in the blood in crucian carp which infected by Aeromonas hydrophila.关键词
盐酸双氟沙星/异育银鲫/嗜水气单胞菌/血浆蛋白结合率/药代动力学Key words
Difloxacin/ Carassais auratus gibebio/ Aerominas hydrophila/ Plasma protein binding rate/ Pharmacokinetic分类
农业科技引用本文复制引用
章海鑫,胡鲲,阮记明,郑卫东,符贵红,杨先乐,王会聪,刘攀..异育银鲫体内盐酸双氟沙星血浆蛋白结合率的变化与其药代动力学研究[J].水生生物学报,2013,37(1):62-69,8.基金项目
国家863计划项目(2011AA10A216) （2011AA10A216）
国家自然科学基金(31172430) （31172430）
农业部行业专项(200803013)资助 （200803013）
