山西医科大学学报2012，Vol.43Issue(12)：911-913,3.DOI:10.3969/J.ISSN.1007-6611.2012.12.007
RT-PCR法检测尿液标本中麻疹病毒核蛋白(N)基因的临床应用及意义
Clinical application and significance of detection of measles virus nucleoprotein(N)gene in urines from patients with measles by RT-PCR
摘要
Abstract
Objective To explore the clinical value of directly detecting measles virus-specific nucleoprotein gene( MV-N )from clinically diagnosed measles patients' urine using reverse transcription-polymerase chain reaction( RT-PCR ). Methods Urine was collected from 15 patients clinically diagnosed with measles from 2010 to 2011 in Bengbu, Anhui province. RNA was extracted from urine. RT-PCR was used to amplify 515 bp nucleotide fragment of the measles virus nucleoprotein. Serum of the same patient was collected to detect MV-IgM antibody. The positive rate was compared between two methods. Results Positive products were successfully amplified in 14 of 15 patients by RT-PCR method. The positive rate was higher by RT-PCR method than by MV-IgM detection method( 93. 3% vs 60. 0%, x2 = 9. 60 ,P = 0. 002 ). Conclusion RT-PCR for detecting the urine can be used as an early fast-assisted diagnosis method for measles virus, especially for patients with early rash. In the meantime, re-examination of negative MV-IgM in serum could reduce the missed diagnosis.关键词
RT-PCR/麻疹病毒/核蛋白基因Key words
RT-PCR/ measles virus/ nucleoprotein gene分类
医药卫生引用本文复制引用
王伟,王海梅,刘双成,郑照军,郑晶,乔艳,钟政荣..RT-PCR法检测尿液标本中麻疹病毒核蛋白(N)基因的临床应用及意义[J].山西医科大学学报,2012,43(12):911-913,3.基金项目
蚌埠医学院科研基金资助项目(BY1036) （BY1036）
