山西医科大学学报2013，Vol.44Issue(2)：87-91,165,6.DOI:10.3969/J.ISSN.1007-6611.2013.02.001
机械创伤通过增加活性氧和NO导致心肌细胞凋亡
Mechanical trauma caused cardiomyocyte apoptosis by increasing the levels of reactive oxygen and nitric oxide
摘要
Abstract
Objective To observe the variation of reactive oxygen and nitric oxide levels after mechanical trauma, and to explore its role in the mechanical trauma-induced cardiomyocyte apoptosis. Methods Mechanical trauma rat model was established by Noble-Collip drum. Myocardial superoxide content was determined by lucigenin-enhanced luminescence. Nitric oxide (NO) content in myocar-dial tissue was detected by chemiluminescene method. Myocardial nitrotyrosine (NT) content was measured by ELISA. Cardiomyocyte apoptosis was confirmed by TUNEL and caspase-3 activity detection. All indices were detected at 0,3,6,12,24 h. FeTMPyP was given immediately after trauma,and the indices were detected and compared at 12 h. Results Cardiomyocyte apoptosis was increased after mechanical trauma (P＜0. 01). Myocardial superoxide and NO content significantly increased after mechanical trauma (P＜0. 01). Peroxynitrite (ONOO- ) ,the product of superoxide and NO, was elevated significantly in myocardial tissue after mechanical trauma ( P ＜ 0. 01). Myocardial NT content and cardiomyocyte apoptosis remarkbly decreased after ONOO scavengers treatment (P ＜ 0. 01) . Conclusion Mechanical trauma could cause the cardiomyocyte apoptosis by increasing the levels of reactive oxygen and nitric oxide in myocardial tissues.关键词
心脏损伤/超氧阴离子/一氧化氮/凋亡/过氧亚硝基Key words
heart injury/ superoxide/ nitric oxide/ apoptosis/ peroxynitrite acid分类
医药卫生引用本文复制引用
燕子,郭丽,杨丽红,卫小红,王洁,王瑾,梁峰..机械创伤通过增加活性氧和NO导致心肌细胞凋亡[J].山西医科大学学报,2013,44(2):87-91,165,6.基金项目
国家自然科学基金资助项目(30872680) （30872680）
山西医科大学细胞生理学省部共建教育部重点实验室主任基金资助项目(2010-10) （2010-10）
山西医科大学博士启动基金资助项目 （）
山西医科大学校青年基金资助项目 （）
