IGF1R-shRNA重组慢病毒的构建及对肺癌A549细胞增殖的影响OACSTPCD
Construction of IGF1R-shRNA lentiviral vector and its effect on proliferation of A549 cells
目的 构建IGF1R基因的shRNA慢病毒载体,转染A549细胞鉴定沉默效率,观察其对A549细胞增殖能力的影响.方法 设计IGF1R干扰序列,与pGC-LV慢病毒载体重组形成shRNA表达载体,包装shRNA慢病毒颗粒,感染A549细胞,应用RT-PCR和Western blot检测IGF1R干扰效果；细胞生长抑制实验、克隆形成实验及细胞周期检测评价其对A549细胞增殖的影响. 结果 成功构建了IGF1R-shRNA重组慢病毒并高效感染A…查看全部>>
Objective To construct the shRNA lentiviral vector targeting IGF1R gene,and to detect the efficiency of gene silence and evaluate its effect on proliferation of A549 cells. Methods DNA oligo was cloned into pGC-LV lentiviral vector to design the IGF1R siRNA targets using short hairpin. ShRNA lentivirus was harvested by virus packaging system. The interference effect of IGF1R on A549 cells was detected by RT-PCR and Western blot. A549 cell proliferation was a…查看全部>>
杜永亮;贾晓民;李海泉;赵杰;王海清
医药卫生
慢病毒短发卡RNAIGF1RA549细胞细胞增殖
lentivirus shRNA IGF1R A549 cell cell proliferation
《山西医科大学学报》 2013 (2)
106-110,167,6
江苏省徐州市科技计划资助项目(XF11C102)
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