山西医科大学学报2013，Vol.44Issue(2)：120-123,168,5.DOI:10.3969/J.ISSN.1007-6611.2013.02.009
辐射对小鼠大脑微血管内皮细胞的作用
Effect of irradiation on cerebral microvascular endothelial cells
摘要
Abstract
Objective To investigate the effects of irradiation on cerebral microvascular endothelial cells and to explore its the potential mechanisms. Methods The primary culture of murine brain microvascular endothelial cells were divided into 3 groups and given Co at the dosage of 0 Gy,l Gy,2 Gy,respectively. Cell morphology and the apoptotic cells of murine brain microvascular endothelial cells were observed by TUNEL method. And the expression of p-ERK and p-JNK was detected by Western blot. Results The apoptosis increased with the increase of radiation dose. The expression of p-ERK decreased at 1Gy radiation dose,and then increased. And the trend of expression of p-JNK was upwards along with the increasing radiation dose. Conclusion The radiation can lead to cell apoptosis, and there are positive correlations between the radiation dosage and the apoptotic rate. MAPK/ERK and MAPK/JNK signal transduction pathways may participate in the regulation of cell apoptosis under radiation conditions.关键词
微血管内皮细胞/辐射/细胞凋亡/ERK/JNKKey words
microvascular endothelial cells/ irradiation/ cell apoptosis/ ERK/ JNK分类
医药卫生引用本文复制引用
李莉,白慧云,赵焕英,丁库克,董小黎..辐射对小鼠大脑微血管内皮细胞的作用[J].山西医科大学学报,2013,44(2):120-123,168,5.基金项目
北京农学院兽医学(中医药)北京市重点实验室2010年度开放课题基金资助项目(BUA-TCVM-201002) （中医药）
