实用医学杂志2012，Vol.28Issue(15)：2490-2492,3.DOI:10.3969/j.issn.1006-5725.2012.15.005
携带Bcl-2基因的慢病毒对磷酰胺氮芥抑制卵巢颗粒细胞体外分泌功能的影响
Lentivirus-mediated Bcl-2 gene expression in ovarian granulose cells improves cell secretion function
摘要
Abstract
Objective To investigate the effect of the lentivirus-mediated Bcl-2 gene expression on apoptosis and secretion function of the chemotherapy-injured human primary ovarian granulosa cells (GCs). Methods Four groups were set in the present experiment: experimental group with pGC-FU-Bcl-2 + PM, empty vector (EV) group with EV + PM, experimental control group with PM, control group only with GCs culture. Apoptosis was detected by DNA-ladder assay. E2 and P in cell culture supernatant were determined by radiation immune assay. Results The obvious DNA ladder bands were shown in agarose gel electrophoresis in experimental control group and EV group, but not in the control group and experimental group. The concentration of E2 and P in experimental group was quite different on day 2 and 5 after adding the drug compared with that in the experimental group and EV group (P < 0.01), but no significant difference in E2 and P concentration was found between the experimental group and EV group (P = 0.990, P= 0.523). Conclusion The lentivirus-mediated Bcl-2 gene expression significantly reduced PM-induced apoptosis of GCs, which could improve secretion function of GCs in vitro.关键词
基因,bcl-2/慢病毒/化疗/分泌功能/原代卵巢颗粒细胞Key words
Genes, bcl-2/ Lentivirus vector/ Drug therapy/ Secretion function/ Ovarian granulose cells引用本文复制引用
王雪峰,何援利,蔡慧华,朱洪磊..携带Bcl-2基因的慢病毒对磷酰胺氮芥抑制卵巢颗粒细胞体外分泌功能的影响[J].实用医学杂志,2012,28(15):2490-2492,3.基金项目
国家自然科学基金资助项目(编号:81041101) （编号:81041101）
广东省自然科学基金资助项目(编号:10451051501004704) （编号:10451051501004704）
