实用医学杂志2013，Vol.29Issue(2)：177-180,4.DOI:10.3969/j.issn.1006-5725.2013.02.005
miR-375在子宫内膜癌HEC-1-B细胞中的生物学功能
The biological function of miR-375 in endometrial carcinoma HEC-1-B cells
摘要
Abstract
Objective To explore the expression of miR-375 in type Ⅱ endometrial carcinoma and its effects on the proliferation and apoptosis of HEC-1-B cells. Methods miRNA expression of type Ⅱ endometrial carcinoma was analyzed by miRNA microarray. miR-375 mimics was transfected into HEC-1-B cells by lipofectamine 2000, then the expression of miR-375 was detected by quantitative real-time PCR. The proliferation was detected by CCK-8, and the apoptosis and transfection efficiency were detected by flow cytometry. Results MiR-375 was down-regulated in type Ⅱ endometrial carcinoma, and the transfection efficiency was 73.43%. The expression of miR-375 in experiment group after transfection was increased than those in NC group and control group (P<0.05). As compared to that in NC group, the inhibitory effect of miR-375 in experiment group was confirmed by CCK-8 (P < 0.05). There was significant difference in the apoptosis rate (P < 0.05) between experiment group and NC group, control group. Conclusion MiR-375 might inhibit the proliferation and promote the apoptosis of type Ⅱ endometrial carcinoma cells, which may be the new target of gene therapy for type Ⅱ endometrial carcinoma.关键词
miR-375/HEC-1-B细胞/增殖/凋亡Key words
MiR-375/ HEC-l-B cells/ Proliferation/Apoptosis引用本文复制引用
叶文蔚,李甫钥,叶夏斌,郑飞云..miR-375在子宫内膜癌HEC-1-B细胞中的生物学功能[J].实用医学杂志,2013,29(2):177-180,4.基金项目
浙江省自然科学基金一般项目(编号:Y2090699) （编号:Y2090699）
