实用医学杂志2013，Vol.29Issue(2)：296-299,4.DOI:10.3969/j.issn.1006-5725.2013.02.055
丙戊酸钠对人脑胶质瘤细胞系SHG-44的放疗增敏作用
Enhancement of human glioma carcinoma cell line SHG-44 radiosensitivity by valproic acid
苏筠 1楚建军 1任峰 1王忠超 1陈秀萍1
作者信息
摘要
Abstract
Objective To investigate radiosensitization effect of valproic acid on human glioma carcinoma cell line SHG-44 and its mechanism. Methods SHG-44 cells were treated with 0.5, 1.0, 2.0, 4.0 and 8.0 mmol/L sodium valproate, respectively. The radio sensitization of valproic acid on SHG-44 cells was measured by colony formation assay. The proliferation inhibition rate of valproic acid on was investigated by MIT assay in different time and dose. The apoptosis and cycle of SHG-44 cells in different groups were analyzed by flow cytometry (FCM). Results After treated with valproic acid, the cell proliferation was inhibited significantly in a time-and dose-dependent manner. The valproic acid have radiosensitization on human glioma carcinoma cell line SHG-44, SER = 1.32. Compared with the control group, cell apoptosis and the proportion of G0/G1 phase were increased significantly in the combination group, and the proportion of S phase was reduced that assayed by FCM. Conclusion Valproic acid can increase the radiosensitization of SHG-44. Its role may be to induced cell apoptosis, change cell cycle phase distribution and other mechanisms.关键词
神经胶质瘤/丙戊酸钠/放疗增敏/细胞凋亡/细胞周期Key words
Glioma/Valproic acid/ Radiosensitization/ Apoptosis/ Cell cycle引用本文复制引用
苏筠,楚建军,任峰,王忠超,陈秀萍..丙戊酸钠对人脑胶质瘤细胞系SHG-44的放疗增敏作用[J].实用医学杂志,2013,29(2):296-299,4.
