天然产物研究与开发2013，Vol.25Issue(1)：31-35,5.
苜蓿素对人直肠癌SW1116细胞增殖和凋亡的作用及其机制
Effects of Tricin on Proliferation and Apoptosis of Human Rectal Cancer SW1116 Cells
摘要
Abstract
In this study, the effects and mechanisms of tricin on proliferation and apoptosis of human rectal cancer SW1116 cells were investigated. MIT assay was used to detect the growth inhibition rates of human rectal cancer SW1116 cells treated with tricin. The apoptosis morphology was observed after AO/EB staining. The cell cycle distribution was measured by flow cytometry. The apoptosis was examined by DNA fragment analysis. The protein expressions of Bcl-2 and Bax were detected by western blot. MTT assay showed that tricin inhibited the growth of SW1116 cells in a dose-dependent manner. The typical apoptotic morphological changes were observed. Tricin blocked SW1116 cells in G0/ G1 and increased the apoptosis rates of SW1116 cells. DNA ladder was observed in cells with the gel imaging analysator. Western blot showed that tricin decreased expression of Bcl-2 and increased expression of Bax in a dose-dependent manner. The results shov: that tricin could inhibit human rectal cancer SW1116 cells proliferation and induce cell cycle block in G0/G1 phase and apoptosis. The down regulation of Bcl-2 and up regulation of Bax protein might be one of the mechanisms.关键词
苜蓿素/直肠癌/增殖/细胞凋亡Key words
tricin/ rectal cancer proliferation/ apoptosis分类
医药卫生引用本文复制引用
陈艳,姚宏,林新华..苜蓿素对人直肠癌SW1116细胞增殖和凋亡的作用及其机制[J].天然产物研究与开发,2013,25(1):31-35,5.基金项目
陈可冀中西医结合发展基金项目 （）
福建省高校中药学重点实验室(福建中医学院)开放课题基金项目(CKJ2007032ZYX200710) （福建中医学院）
福建省教育厅科研基金项目(JA07101) （JA07101）
福建省自然科学基金项目(2009J01157) （2009J01157）
