天然产物研究与开发2013，Vol.25Issue(1)：92-95,100,5.
中压制备液相色谱快速分离制备儿茶素单体
Separation and Preparation of Catechins by Preparative Medium Pressure Liquid Chromatography
摘要
Abstract
With advances in column particles,preparative medium pressure Liquid chromatography (MPLC) is an potential alternative to preparative HPLC for purifying active botanical compounds on reverse phase. To obtain high-purity cat-echin monomers, a MPLC system with online real-time operation software (DR FLASH Ⅱ) was introduced to separate catechin monomers from tea polyphenols with the methanol-water elution on two serial connected C18 (ODS-AQ) columns (125 mm × 26 mm, 30-50 μm,40 g) at 120-150 psi. With loading of 1. 0 g of tea polyphenols with the purity of 92. 6% , the four catechin monomers as epigallocatechin ( EGC), epigallocatechin gallate ( EGCG), epicatechin (EC) and epicatechin gallate (ECG) were separated and purified at only one step in one time. 90 mg of EGC,355 mg of EGCG,23 mg of EC ,92 mg of ECG were harvested and their purities were 91. 8% ,97.6% ,97.7% and 99.3% respectively. The yields and recovery of total four catechins monomers were up to 56.0% and 68.2% respectively. The structures of the four catechins were subsequently confirmed by analysis of their H NMR,13C NMR and HRESI-MS. Those indicate that MPLC could be helpful to the rapid and efficient preparation of catechin monomers from tea polyphenols.关键词
儿茶素单体/中压制备液相色谱/核磁共振谱/高分辨质谱Key words
catechin monomers/preparative medium pressure liquid chromatography/NMR/HRESI-MS分类
化学化工引用本文复制引用
林丹,李春苗,鲜殊,王玺,宛晓春,凌铁军,李大祥..中压制备液相色谱快速分离制备儿茶素单体[J].天然产物研究与开发,2013,25(1):92-95,100,5.基金项目
安徽省国际科技合作计划项目(10080703036) （10080703036）
现代农业(茶叶)产业技术体系建设专项资金(农科教发[2011]3号) （茶叶）
