西安交通大学学报（医学版）2013，Vol.34Issue(2)：155-158,172,5.
siRNA沉默STAT3基因对肝癌细胞HepG2增殖的影响
siRNA against signal transducer and activator of transcription 3 inhibits proliferation of HepG2 cells in vitro
摘要
Abstract
Objective To study the proliferation change of HepG2 cells by inhibiting the expression of signal transducer and activator of transcription 3 (STAT3) in vitro and to investigate its underlying molecular mechanism. Methods siRNA against STAT3 inhibited the expression and activation of STAT3 protein in HepG2 cells in vitro; cell proliferation and cycle were detected by MTT assay and flow cytometry respectively; transcriptions of survivin and caspase3 were detected by reverse transcription PCR. Results Proliferation of HepG2 cells was inhibited by blocking JAK2/STAT3 signaling pathway; cell division was arrested at G0-G1 phase. Transcription of survivin was downregulated while that of caspase3 was upregulated. Conclusion Proliferation and division of HepG2 cells can be inhibited by blocking JAK2/STAT3 signaling pathway, the mechanism of which may be related to be regulation of survivin and caspase3.关键词
原发性肝癌/信号转导和转录活化因子3/RNA干扰/JAK2/STAT3信号通路Key words
primary hepatocellular carcinoma/ signal transducer and activator of transcription 3 (STAT3)/ RNA interference/ JAK2/STAT3分类
医药卫生引用本文复制引用
宋涛,王艳丽,杨雪,刘青光..siRNA沉默STAT3基因对肝癌细胞HepG2增殖的影响[J].西安交通大学学报（医学版）,2013,34(2):155-158,172,5.基金项目
陕西省科技攻关资助项目(No.2010K01-131),西安交通大学医学创新基金资助项目(No.GH0203108) （No.2010K01-131）
