西安交通大学学报（医学版）2013，Vol.34Issue(2)：159-163,5.
高迁移率族蛋白1对人肝癌细胞增殖及转移能力的影响
Effects of HMGB1 on proliferative and metastatic abilities of hepatoma cell line HepG2
摘要
Abstract
Objective To investigate effects of high mobility group box-1 (HMGB1) on the proliferative and metastatic abilities of hepatoma cell line HepG2 in vitro and analyze the possible mechanisms. Methods MTT was used to determine the proliferative and adhesive abilities of HepG2. The invasive and migratory abilities were detected by Transwell assay. The protein and mRNA expressions of matrix metalloproteinases-2 (MMP-2), matrix metalloproteinases-9(MMP-9) and vascular endothelial growth factor (VEGF) were evaluated by Western blot and reverse transcription (RT-PCR), respectively. Results The proliferative ability of HepG2 in response to rHMGBl of different concentrations (10, 100 and 1 000 ng/mL) was significantly higher than that in the control group at 24, 48 and 72h (P<0.01), and it increased with the increase of treatment concentration and time. HepG2 was treated by rHMGBl (100 ng/mL) for 48 h to detect the adhesive, invasive and migratory abilities. Compared to the control group, the adhesive ability of HepG2 to Matrigel was significantly elevated (P<0.01) and the number of invasive and migratory cells was remarkably increased (P<0. 01). In addition, the protein and mRNA expressions of MMP-9 and VEGF were significantly up-regulated (P<0.01), but there was no difference in the protein and mRNA expressions of MMP-2 (P>0.05). Conclusion The proliferative, adhesive, invasive and migratory abilities of hepatoma cells can be promoted by HMGB1, which may be related to up-regulating the protein and mRNA expressions of MMP-9 and VEGF.关键词
高迁移率族蛋白1(HMGB1)/肝癌细胞/增殖/肿瘤转移/基质金属蛋白酶2(MMP-2)/基质金属蛋白酶9(MMP-9)/血管内皮生长因子(VEGF)Key words
high mobility group box-1 (HMGB1)/ hepatoma cell/ proliferation/ tumor metastasis/ matrix met-alloproteinases-2(MMP-2)/ matrix metalloproteinases-9(MMP-9)/ vascular endothelial growth factor (VEGF)分类
医药卫生引用本文复制引用
刘亚萍,董蕾,史海涛,刘欣,鲁晓岚,姜炅..高迁移率族蛋白1对人肝癌细胞增殖及转移能力的影响[J].西安交通大学学报（医学版）,2013,34(2):159-163,5.基金项目
国家自然科学基金青年基金资助项目(No.81200310) （No.81200310）
