西安交通大学学报（医学版）2013，Vol.34Issue(2)：177-180,4.
低硒大鼠心肌线粒体CytC含量的动态变化及其在心肌细胞凋亡中的作用
The dynamic change of CytC expression in cardiac mitochondria in selenium deficiency rats and its effect on myocardial apoptosis
摘要
Abstract
Objective To investigate the expression of cytochrome C (CytC) protein in myocardial mitochondria in selenium deficiency rats and the apoptosis of myocardial cells so as to discuss the effect of CytC in selenium deficiency on myocardial apoptosis. Methods The model of selenium deficiency was established in two-week-old rats. When the rats were fed for 20 week, 30 week and 40 week, the myocardial mitochondria of myocardial cells was extracted to assay the expression of mitochondrial CytC protein by Western blot and to detect the apoptosis of myocardial cells by immunohistochemistry (SP method). We then tested the index of myocardial cells and analyzed the correlation between CytC and apoptosis index of myocardial cells. Results The expression of CytC protein in low-selenium (LS) groups was decreased significantly compared with that in normal control (NC) groups of corresponding time point (P<0.05); as low-selenium feeding time prolonged, it was reduced significantly in LS subgroups (P<0.05). However, there was no significant difference between NC subgroups (P>0.05). With the prolonging of low-selenium feeding time, the number of apoptotic myocardial cells in LS group was increased. The correlation analysis indicated that mitochondrial CytC was negatively correlated with the apoptosis index of myocardial cells (rs = - 0. 858, P<0. 01). Conclusion Selenium deficiency can affect the expression of mitochondrial CytC protein in rats. CytC is correlated with the apoptosis index of myocardial cells, suggesting that it plays an important role in the mitochondria-mediated apoptosis of myocardial cells.关键词
心肌线粒体/细胞色素C/凋亡/低硒/心肌细胞凋亡Key words
myocardial mitochondria/ cytochrome C (CtyC)/ apoptosis/ selenium deficiency/ myocardial cells/ apoptosis分类
医药卫生引用本文复制引用
潘晓青,魏瑾,林琳,张明,单虎,闫蕊,贺乐..低硒大鼠心肌线粒体CytC含量的动态变化及其在心肌细胞凋亡中的作用[J].西安交通大学学报（医学版）,2013,34(2):177-180,4.基金项目
国家自然科学基金资助项目(No.30771862),陕西省科技攻关项目(No.2007K13-03(14)),陕西省自然科学基金资助项目(No.2003C225) （No.30771862）
