西安交通大学学报（医学版）2013，Vol.34Issue(2)：248-252,5.
大黄素对急性放射性肠炎肠黏膜屏障的保护作用
Protective effects of emodin on intestinal mucosal barrier in acute radiation enteritis
摘要
Abstract
Objective To investigate the effects and mechanisms of emodin on acute radiation enteritis models and intestinal mucosal barrier. Methods Totally 50 healthy male SD rats were randomly divided into normal group, model group, emodin prevention group, emodin treatment group and SiMiDa group. Emodin prevention group had been given emodin for 3 consecutive days in advance. Except normal group, the others were given a single dose of 10 Gy 6 MV of higher-energy X-rays on the abdominal region to establish acute radiation enteritis models. After 6 h, emodin prevention group, emodin treatment group and SiMiDa group were given intragastric administration for 4 days. The morphologic indexes were measured by light microscopy and the image analysis system. Intestinal diamine oxidase (DAO) activities as well as bacteria translocation rates of liver, spleen and mesenteric lymph node were measured. Intestinal tumor necrosis factor alpha (TNF-α) was measured by ELISA and nitric oxide (NO) level was measured by spectrophotometer. Results Emodin prevention group, emodin treatment group and SiMiDa group had significantly higher levels of villus height, crypt depth, thickness of mucosa and entire wall as well as DAO activities than model group (P<0.05). Emodin prevention group, emodin treatment group and SiMiDa group had significantly decreased bacteria translocation rates of liver, spleen and mesenteric lymph node as well as TNF-α activities and NO levels (P<0. 05). There were no differences between emodin prevention group and emodin treatment group (P>0.05). Conclusion Emodin could significantly increase villus height of the small intestine, crypt depth and mucosal layer thickness, enhance intestinal tissue DAO activities, protect the intestinal mucosa, reduce bacterial translocation rate, and reduce TNF-α expression in the intestinal tissue and NO generation. There are no significant differences between prophylaxis and treatment administration of emodin.关键词
急性放射性肠炎/大黄素/二胺氧化酶/肿瘤坏死因子/一氧化氮/细菌移位率/肠黏膜屏障保护Key words
acute radiation enteritis/ emodin/ diamine oxidase/ tumor necrosis factor alpha (TNF-α)/ nitric oxide (NO)/ bacterial translocation rate/ intestinal mucosal barrier分类
医药卫生引用本文复制引用
王玉,周冬枝,夏欣欣,韩萍萍,曹丽君..大黄素对急性放射性肠炎肠黏膜屏障的保护作用[J].西安交通大学学报（医学版）,2013,34(2):248-252,5.基金项目
陕西省中医药管理局资助项目(No.JC-33) （No.JC-33）
