西北林学院学报2013，Vol.28Issue(2)：83-89,7.DOI:10.3969/j.issn.1001-7461.2013.02.16
假臭草ISSR-PCR反应体系的建立与优化
Establishment and Optimization of ISSR-PCR Reaction System for Eupatorium catarium
摘要
Abstract
The effects of 5 factors ( Mg2+ concentration, dNTPs concentration, Taq DNA polymerase dosage, primer concentration and DNA templates concentration) on ISSR amplification were tested, and the annealing temperature was selected and optimized for establishment of a suitable ISSR-PCR reaction system of Eupatorium catarium through single factor test and orthogonal experiment design of L16 (4°). The results showed that the optimal ISSR-PCR reaction system (20 μL) contained 2. 0 mmol/L Mg2+,60 ng DNA template, 1. 5 U Taq DNA polymerase,200 μmol/L dNTPs and 0. 3 jumol/L primer. The establishment of the ISSR-PCR reaction system could lay a favorable foundation for the analysis of the genetic diversity of E. Catarium and for the exploration of prevention and control strategy for E. Catarium.关键词
假臭草/ISSR/反应体系/优化Key words
Eupatorium catarium/ ISSR/ reaction system/ optimization分类
农业科技引用本文复制引用
黎丽倩,李妮亚,刘强,王婷,杨丽,石俊菲..假臭草ISSR-PCR反应体系的建立与优化[J].西北林学院学报,2013,28(2):83-89,7.基金项目
国家自然科学基金项目(31060095). （31060095）
