医药导报2013，Vol.32Issue(2)：212-214,3.DOI:10.3870/yydb.2013.02.025
薄层色谱法测定亚甲蓝有关物质的稳定性考察
Determination of Stability of Related Substances in Methylene Blue by TLC
楼永军1
作者信息
- 1. 浙江省食品药品检验研究院化学药品室,杭州,310004
- 折叠
摘要
Abstract
Objective To develop a thin-layer chromatography (TLC) method for determination of related substances in methylene blue and investigate the stability of it. Methods Five uL of test solution was applied to a suitable thin-layer
chamber with a solvent system consisting of the upper layer separated from a well-shaken mixture of water, n-butanol and glacial acetic acid ( 100 : 80 : 20 ) , and examined under ultraviolet light at 254 nm and natural light. Results Methylene blue underwent extensive degradation under thermal stress (60℃ ) . It was found to degrade under thermal stress (40℃) and photolytic conditions. It possessed hygroscopicity and degraded under high humidity conditions ( RH 92. 5% and 15% ) . Conclusion The studies show that methylene blue is hygroscopic and unstable.关键词
亚甲蓝/色谱法,薄层/有关物质/稳定性Key words
Methylene blue/Thin-layer chromatography/Related substances/Stability分类
医药卫生引用本文复制引用
楼永军..薄层色谱法测定亚甲蓝有关物质的稳定性考察[J].医药导报,2013,32(2):212-214,3.
