应用生态学报2012，Vol.23Issue(3)：791-797,7.
蠋蝽抗寒性对快速冷驯化的响应及其生理机制
Responses of Arma chinensis cold tolerance to rapid cold hardening and underlying physiological mechanisms
摘要
Abstract
Rapid cold hardening can enhance the cold tolerance of some insects. To explore the effects of different cold hardening induction temperature on the cold tolerance of Arma chinensis and related physiological mechanisms, the 3rd generation A. chinensis adults reared indoor were treated with cooling at 15, 10, and 4 ℃ for 4 h, respectively, or with gradual cooling from 15 ℃ for 4 h to 10 ℃ for 4 h, and finally to 4 ℃ for 4 h. The super-cooling point, water content, and the contents of low molecular carbohydrates, glycerol, and amino acids of the adults after cooling and the adults cold tolerance at 0, -5, and -10 ℃ were measured by thermocouple, high performance liquid chromatography, and other analytical techniques. When exposed at -10℃ after cooling, the survival rate of the adults treated with gradual cooling or treated with cooling at 4℃for 4 h was aver-agely 58. 3% , while that of the adults reared at room temperature (25 ℃ ±2 ℃ ) or treated with cooling at 15 ℃ or 10 ℃ for 4 h decreased significantly, with an average of 8. 9%. The super-cooling point of the adults treated with gradual cooling or with cooling at 4 ℃ for 4 h was -15. 6 ℃ , which was averagely 1.3 ℃ lower than that of the other treatments. The water content of the adults had no significant difference among all treatments, with an average of 61.8% , but the glucose, sor-bitolum, glycerol, Ala, and Glu contents in treatments gradual cooling and cooling at 4 ℃ for 4 h increased by 2.82-fold, 2.65-fold, 3.49-fold, 51.3% , and 80.2% , while the fucose, mannose, and Pro contents decreased by 68.4% , 52.2% , and 30.2% , respectively, as compared with the other treatments. The fructose content showed no significant difference among all treatments. It was suggested that rapid cool hardening had a critical temperature to induce the physiological metabolism process of adult A. chinensis, and gradual cooling hardening could not further increase the cold tolerance of adult A. chinensis on the basis of rapid cool hardening.关键词
蠋蝽/快速冷驯化/抗寒性/生理机制Key words
Arma chinensis/ rapid cold hardening/ cold tolerance/ physiological mechanism分类
生物科学引用本文复制引用
李兴鹏,宋丽文,张宏浩,陈越渠,左彤彤,王君,孙伟..蠋蝽抗寒性对快速冷驯化的响应及其生理机制[J].应用生态学报,2012,23(3):791-797,7.基金项目
国家林业局"948"项目(2008-4-59)和吉林省科技发展计划项目(20100158)资助. （2008-4-59）
