应用生态学报2012，Vol.23Issue(12)：3441-3448,8.
长江口低氧区沉积物表层纤毛虫多样性及其时空变化
Ciliate diversity and spatiotemporal variation in surface sediments of Yangtze River estuary hypoxic zone
摘要
Abstract
By using denaturing gradient gel electrophoresis ( DGGE ) and sequencing as well as Lu-dox-QPS method, an investigation was made on the ciliate diversity and its spatiotemporal variation in the surface sediments at three sites of Yangtze River estuary hypoxic zone in April and August 2011. The ANOSIM analysis indicated that the ciliate diversity had significant difference among the sites (R = 0.896, P = 0.0001), but less difference among seasons (R = 0. 043, P = 0. 207). The sequencing of 18S rDNA DGGE bands revealed that the most predominant groups were planktonic Choreotrichia and Oligotrichia. The detection by Ludox-QPS method showed that the species number and abundance of active ciliates were maintained at a higher level, and increased by 2—5 times in summer, as compared with those in spring. Both the Ludox-QPS method and the DGGE technique detected that the ciliate diversity at the three sites had the similar variation trend, and the Ludox-QPS method detected that there was a significant variation in the ciliate species number and abundance between different seasons. The species number detected by Ludox-QPS method was higher than that detected by DGGE bands. Our study indicated that the ciliates in Yangtze River estuary hypoxic zone had higher diversity and abundance, with the potential to supply food for the polyps of jellyfish.关键词
纤毛虫多样性/长江口低氧区/DGGE指纹图谱/水母暴发Key words
ciliate diversity/ hypoxic zone of Yangtze River estuary/ DGGE fingerprinting/ jellyfish bloom分类
生物科学引用本文复制引用
赵峰,徐奎栋,孟昭翠..长江口低氧区沉积物表层纤毛虫多样性及其时空变化[J].应用生态学报,2012,23(12):3441-3448,8.基金项目
国家重点基础研究发展计划项目(2011CB403604)和中国科学院知识创新工程重要方向项目(KSCX2-EW-Z-5)资助. （2011CB403604）
