诊断学理论与实践2013，Vol.12Issue(1)：38-42,5.DOI:10.3969/j.issn.1671-2870.2013.01.009
同步辐射微血管成像技术在动物模型中的应用初探
Use of synchrotron radiation microvascular imaging technique in animal model: a preliminary investigation
摘要
Abstract
Objective Using synchrotron radiation source to establish an effective in vivo dynamic imaging technique with high resolution brain vasculature in rats. Methods SD rats weighing 300-350 g were used. Two kinds of contrast agent (iodine and barium sulfate contrast agent) were injected through an intracarotid arterial cannula, and by adjusting the imaging parameters to observe brain microvascular structure under normal physiological condition in situ and in vivo, thereby to define the parameter criteria needed for imaging. Results Barium sulfate was suitable for observing the blood vessel in situ in rats, and non-ionic iodine contrast agent could be used for real time in vivo brain vascular imaging in rats. Through experiment the imaging parameters needed for in vivo imaging in rats were established; time subtraction method can reduce the influence of background and bone on the imaging obtained. Conclusions Non-ionic iodine contrast agent is suitable for in vivo microvascular imaging in rats. Synchrotron radiation source is a high resolution imaging technique for observing in vivo the microvascular structure in experiment animals, providing a new technique and approach for studying effectively the brain vascular structure in vivo.关键词
同步辐射/血管成像/脑血管/大鼠Key words
Synchrotron radiation/ Angiography/ Brain vessels/ Rats分类
医药卫生引用本文复制引用
管永靖,陈克敏,袁法磊,王永亭,任玉琦,肖体乔,杨国源,凌华威..同步辐射微血管成像技术在动物模型中的应用初探[J].诊断学理论与实践,2013,12(1):38-42,5.基金项目
上海市重点学科建设项目"影像医学与核医学"(S30203) （S30203）
国家重点基础研究发展计划(973计划)"基于上海光源针对重大疾病医学影像的若干关键问题研究"(2010CB834306) （973计划）
