诊断学理论与实践2013，Vol.12Issue(1)：47-51,5.DOI:10.3969/j.issn.1671-2870.2013.01.011
高密度脂蛋白中的1-磷酸鞘氨醇对心肌细胞生存信号通路的影响
Effect of sphingosine-1-phosphate (S1P) in HDL on cardiomyocyte survival signaling pathway
摘要
Abstract
Objective By observing the influence of sphingosine-1-phosphate (S1P) in various components of HDL on cardiomyocyte AKT and ERK1/2 signaling pathway to investigate the protective effect of HDL on cardiomyocytes. Methods Different components of HDL were separated by ultracentrifugation and chromatographic analysis. The content of S1P in various components of HDL was detected by digoxin nucleotide labeling method . Mouse cardiomyocytes were treated with different HDL components and VPC23019 (a S1P1, and S1P3 receptor antagonist). Western blot was used to detect the change of phosphorylated AKT and ERK 1/2 signaling pathway. Results Compared with control group, the level of phosphorylated AKT and ERK 1/2 increased after stimulated with different HDL components,and the level of increase was correlated with the content of S1P in different HDL components. This effect was abrogated by VPC 23019. Conclusions Various components of HDL could activate PI3K-AKT and MEK-ERK1/2 signaling pathway via the S1P receptor on cell membrane. This effect was completely inhibited by S1P1 and S1P3 receptor antagonist VPC23019. Myocardial protection effect of HDL might be through S1P and was mediated by S1P1 and S1P3 receptors.关键词
1-磷酸鞘氨醇/高密度脂蛋白/VPC23019/信号通路Key words
Sphingosine-1-phosphate/ High-density lipoprotein/ VPC23019/ Signaling pathway分类
医药卫生引用本文复制引用
王敏,倪熠,陈秋静,陆林,陶蓉..高密度脂蛋白中的1-磷酸鞘氨醇对心肌细胞生存信号通路的影响[J].诊断学理论与实践,2013,12(1):47-51,5.基金项目
国家自然科学基金面上项目(81070175) （81070175）
上海市科委基础研究重点项目(12JC1406300) （12JC1406300）
