诊断学理论与实践2013，Vol.12Issue(1)：80-85,6.DOI:10.3969/j.issn.1671-2870.2013.01.018
实验性软骨损伤的生物学表达与病理分期对照研究
Biological marker expression and pathological staging of experimental cartilage injury
摘要
Abstract
Objective To observe the pathological staging and the relation between staging and expression of cytokines in experimental cartilage injury (experimental osteoarthritis). Methods Knee joint cartilage injury model was constructed in 18 New Zealand white rabbits and the rabbits were categorized into 2-, 4-, 8-, 12-, 16-, 20-week groups (3 rabbits in each group). Another 6 rabbits were served as controls (sham operation). Serum BMP-2 (bone morphogenetic protein-2), CTX- Ⅱ ( type Ⅱ collagen C-terminal peptide), and COMP (cartilage oligomeric matrix protein) were determined, and rabbits were sacrificed at the end of experiment with their left femur condyle cartilage taken for gross and HE stain microscopic examination. Pathologic scores were assessed in accordance with the Mankin score evaluation system. Results Serum BMP-2 level was elevated by 79.86, 62.74, 53.34, 31.41, 47.82, 13.05 pg/mL at 2, 4, 8, 12, 16, 20 weeks after construction of model, the difference with controls was statistically significant. Serum level of CTX- Ⅱ was elevated by 0.075, 0.008, 0.280, 0.336, 0.357, 0.051 ng/mL at 2, 4, 8, 12, 16, 20 week time node, the difference with controls was statistically significant except that at 4 week time node (P=0.43). The level of COMP in serum was elevated by 0.554, 0.528, 0.612, 0.926, 0.217 ng/mL at the time node of 2, 4, 8, 12, 16 week, the difference was statistically significant when compared with control; COMP level at 20 week was not significantly different from that of control. HE stain microscopic examination demonstrated that the ratio and extent of cartilage injury increased with the prolongation of time. Mankin Scores were: 0.33 (pre-operation), 2.00 ( 2 weeks), 4.33 ( 4 weeks) ,7.67 ( 8 weeks), 9.33 (12 weeks), 11.33 (16 weeks), 13.00 ( 20 weeks). Conclusions The animal model designed in this study can replicate the early, middle and late stage cartilage change in osteoarthritis, and the serum levels of BMP-2, CTX-Ⅱ, COMP can reflect indirectly the extent of cartilage injury.关键词
关节软骨/动物模型/骨形态发生蛋白2/Ⅱ型胶原C端肽/软骨寡聚基质蛋白/Mankin评分Key words
Articular cartilage/Animal model/Bone morphogenetic protein-2/ Type Ⅱ collagen C-terminal peptide/ Cartilage oligomeric matrix protein/ Mankin Score分类
医药卫生引用本文复制引用
姜铃霞,姚伟武,赵海南,杨世埙..实验性软骨损伤的生物学表达与病理分期对照研究[J].诊断学理论与实践,2013,12(1):80-85,6.基金项目
国家自然科学基金面上项目(81171312) （81171312）
上海市科委医学引导项目(1041196600) （1041196600）
