诊断学理论与实践2013，Vol.12Issue(1)：86-89,4.DOI:10.3969/j.issn.1671-2870.2013.01.019
骨肉瘤PTEN启动子特定区域甲基化的研究
Methylation of PTEN promoter specific domain in osteosarcoma
摘要
Abstract
Objective To investigate the promoter CpG islands methylation status of tumor suppressor gene PTEN in osteosarcoma and to investigate the function of PTEN in the pathogenesis of osteosarcoma. Methods Forty-three cases of osteosarcoma were collected. The CpG islands methylation ratio of PTEN promoter 003 domain were detected by MALDI-TOF MS in 32 cases of osteosarcoma and blood samples of 17 normal individuals. Results Forty-three eases of osteosarcoma comprised 20 male (46.5%) and 23 female (53.5%); male to female ratio was 1:1.15. The ages of patients ranged from 9-74 years, with a median age of 25 and mean age of 30. 20.9% of the osteosarcoma were located in the distal femur, 20.9% in the proximal tibia and 16.3% in distal humerus. The methylation ratio of CpG_l, CpG_3.4, CpG_9, CpG_11.12,CpG_13 and CpG_18.19.20 in PTEN promoter 003 domain was significantly higher than that of normal blood sample control (P<0.05). Conclusions High methylation ratio of PTEN promoter may cause the loss of PTEN function and be involved in the pathogenesis of osteosarcoma.关键词
骨肉瘤/人第10号染色体缺失的磷酸酶及张力蛋白同源的基因/抑癌基因/甲基化Key words
Osteosarcoma/ PTEN/ Tumor suppressor gene/ Methylation分类
医药卫生引用本文复制引用
何博,李锋,蒋金芳,刘伟,朱慧敏,孙丽丽,尹亮,梁伟华,常彬..骨肉瘤PTEN启动子特定区域甲基化的研究[J].诊断学理论与实践,2013,12(1):86-89,4.基金项目
国家自然科学基金-西部项目(30560169). （30560169）
