中国临床药理学与治疗学2013，Vol.18Issue(2)：127-131,5.
酚红外排水平评价肺上皮细胞多药耐药相关蛋白1的功能
Evaluation on the function of MRP1 in pulmonary epithelial cells by the efflux level of phenolsulfonphthalein
摘要
Abstract
AIM: To evaluate the function of MRP1 in pulmonary epithelial cells by the efflux level of substrate using phenolsulfonphthalein as MRP1 substrate and probenecid as MRPl-specif ic inhibitor.METHODS: The mouse was divided into the control group and inhibitor inhaled groups.The bronchoalveolar lavage fluid (BALF) and plasma samples were collected at 30 min after phenolsulfonphthalein (250 mg/kg) was administered to the control group mouse by intravenous injection.The inhibitor inhaled groups mouse was administered phenolsulfonph thalein (250 mg/kg) at 10, 30 and 60 min after inhaled probenecid (150 mg/kg), and the BALF and plasma samples were collected at 30 min af ter phenolsulfonphthalein administration.The phenolsulfonphthalein concentration in BALF and plasma was determined and the concentra tion of in BALF/plasma ratio was calculated.RESULTS: There was no difference in the plasma concentration of phenolsulfonphthalein between the control group and inhibitor groups.Com pared with control group, the phenolsulfonph thalein concentration in BALF and the phenol sulfonphthalein concentration of in BALF/plas ma ratio of the 60 min after inhaled probenecid group had significant difference.CONCLUSION: The efflux level of phenolsulfonphthalein in BALF could reflect the function of mouse pul monary epithelial cells MRP1.关键词
酚红/外排水平/多药耐药相关蛋白1/丙磺舒Key words
Phenolsulfonphthalein/Efflux level/MRP1/Probenecid分类
医药卫生引用本文复制引用
陶秀华,汪电雷,曹银,汪辰吟,陈金佩,杨丽丽..酚红外排水平评价肺上皮细胞多药耐药相关蛋白1的功能[J].中国临床药理学与治疗学,2013,18(2):127-131,5.基金项目
国家自然科学基金(81001592) （81001592）
教育部科学技术重点项目资助(210101) （210101）
安徽高校省级自然科学研究重点项目资助(KJ2010A210) （KJ2010A210）
