中国临床药理学与治疗学2013，Vol.18Issue(2)：156-160,5.
马齿苋总黄酮对血小板源性生长因子致家兔血管平滑肌细胞增殖的抑制作用
Effects of portulaca total flavone on proliferation of vascular smoothmuscle stimulated by PDGF-BB
摘要
Abstract
AIM: To study the effects of por tulaca total flavone (PTF) on proliferation in duced by PDGF-BB of rabbit aortic smooth mus cle cells (VSMCs) and explore its mechanisms.METHODS: The VSMCs of cultured organiza tion-adherence was induced to proliferate by PDGF-BB, and effects of PTF on VSMCs was tested by cell counting method and 3H-thymi dine (3H-TdR) incorporation test;The effect of PTF on cell proliferative cycle of VSMC was ob served by flow cytomytry technique;The intra cellular calcium concentration was investigated by fluorimetry.RESULTS: PTF inhibited the proliferation of VSMCs induced by PDGF-BB in a dose dependent manner, and DNA symthesis, the cells numbers of G1 /G0 phase were increased and that of G2/S phase were decreased markedly (P<0.01).CONCLUSION: Proliferation of VSMCs induced by PDGF-BB can be inhibited by PTF.The mechanisms may be related with di minished VSMCs [Ca2+]i.关键词
马齿苋总黄酮/血管平滑肌细胞/细胞增殖/血小板源性生长因子-BB型/氚-胸腺嘧啶核苷/细胞周期/细胞内钙Key words
Portulaca total flavone/Vascular smooth muscle cslls/Cell proliferation/PDGFBB/3H-TdR/Cell cycle/[ Ca2+]i分类
医药卫生引用本文复制引用
卢新华,黄煌,谭斌,邓华菲,王桂霞,左克强,李坚,何军山..马齿苋总黄酮对血小板源性生长因子致家兔血管平滑肌细胞增殖的抑制作用[J].中国临床药理学与治疗学,2013,18(2):156-160,5.基金项目
湖南省自然科学基金项目(06JJ50064) （06JJ50064）
湖南省教育厅十二·五重点建设学科基金资助项目编号 （）
湖南省心脑血管天然药物研究重点实验室基金项目资助 （）
