中国全科医学2013，Vol.16Issue(9)：1004-1007,1011,5.DOI:10.3969/j.issn.1007-9572.2013.03.089
塞来昔布联合多西他赛抑制人乳腺癌MDA-MB-231细胞增殖和诱导凋亡的作用研究
Celecoxib and Docetaxel Inhibited Proliferation and Induced Apoptosis of Breast Cancer Cell Line MDA-MB-231
摘要
Abstract
Objective To investigate the effect of celecoxib and docetaxel on the proliferation and apoptosis of breast cancer cell line MDA - MB - 231. Methods Breast cancer cell line MDA - MB - 231 was treated by 60 μmol/L celecoxib ( celecoxib group ), 2. 0 μmol/L docetaxel ( docetaxel group ) and 60 μmol/L celecoxib plus 2. 0 μmol/L docetaxel ( combination group ) respectively. The cell line was cultivated for 24 h, 48 h and 72 h respectively. MTT method was used to detect cell survival rate, Wright - Giemsa staining was used to observe morphological changes, Annexin - V/PI flow cytometry was used to analyze cell apoptosis and Western Blot method was used to detect the expression of bcl - 2, bax and survivin. Results The cell survival rate of MDA - MB - 231 cell line between celecoxib group, docetaxel group and combination group showed statistically significant difference ( Fbetween groups = 9. 78, P = 0. 03, Ftime = 10. 45 , P = 0. 03 ) . The CDI was ( 0. 46 ± 0. 21 ), ( 0. 23 ± 0. 14 ) and ( 0. 17 ± 0. 04 ) respectively after 24 h, 48 h and 72 h action on cells in the combination group. In Wright - Giemsa staining, under 400 times lens, cells in celecoxib group and docetaxel group showed double nucleuses and stopped in metaphase, while cells in combination group showed chromatin condensation, fragmented nucleus and apoptosis body. After 48 h action on cells, the apoptosis rate of celecoxib group, docetaxel group and combination group was ( 20. 14 ± 1. 22 ) % , ( 54. 60 ± 1. 37 ) % and ( 59. 85 ± 1. 42 ) % , and the difference was statistically significant ( F = 1 395. 96, P =0. 00 ) . The results of Western Blot showed that after 48 h action on cells, the expression of bcl - 2 in celecoxib group, docetaxel group and combination group decreased to 89. 02% , 75. 63% and 45. 35% of the control group respectively, and the difference was statistically significant (x2 = 13. 77 , P < 0. 05 ) . The expression of bax increased to 136. 35% , 157. 24% and 173. 89% of the control group and the difference was statistically significant (x2 =4. 79, P <0. 05 ) . And the expression of surviving decreased to 80. 42% , 54. 28% and 35. 85% of the control group in the three groups and the difference was statistically significant ( x 2 = 10. 28, P < 0. 05 ). Conclusion Celecoxib and docetaxel have synergistic effect on growth inhibition and apoptosis of breast cancer MDA - MB - 231 cells and its mechanism may be related to the decreasing of bcl - 2, survivin expression and increasing of bax expression.关键词
塞来昔布/多西他赛/乳腺肿瘤/细胞凋亡Key words
Celecoxib/ Docetaxel/ Breast neoplasms/ Apoptosis分类
医药卫生引用本文复制引用
许燕艳,翟凤钰,王亚帝,岳莉,漆起贵,王芳..塞来昔布联合多西他赛抑制人乳腺癌MDA-MB-231细胞增殖和诱导凋亡的作用研究[J].中国全科医学,2013,16(9):1004-1007,1011,5.基金项目
CSCO-金港榄香烯肿瘤学研究生课题基金(z-2011-004) （z-2011-004）
