中国医学科学院学报2013，Vol.35Issue(1)：1-5,5.DOI:10.3881/j.issn.1000-503X.2013.01.001
肝细胞生长因子对缺氧损伤的肺微血管内皮细胞的保护作用
Protective Effects of Hepatocyte Growth Factor on Hypoxic Human Pulmonary Microvascular Endothelial Cells
摘要
Abstract
Objective To investigate the protective effects of hepatocyte growth factor ( HGF) on hy-poxic human pulmonary microvascular endothelial cells ( HPMECs). Methods HPMECs were cultured in vitro, and the hypoxic model was established by the physical method. Cells were divided into 4 groups; the control group, the hypoxic group, HGF group, and phytohemagglutinin ( PHA) group. The 7th generation of HPMECs was evaluated by the method of immunocytochemistry. The persistence rate of HPMECs was measured by MTT assay and the adhesive cells were counted by the microscopy. The expression of intercellular adhesion molecule-l ( ICAM-1 ) protein was determined by immunofluorescence staining. Results The adherence percentage of cells significantly decreased after hypoxia, whereas the expression of the ICAM-1 protein was sig-nificantly higher in the hypoxia group than in control group ( P < 0. 01). Compared with the hypoxia group, the persistence and adherence percentage of cells in the HGF group significantly increased (P < 0. 01) , whereas the expression of the ICAM-1 protein significantly dropped (P <0. 01). In the PHA group, the persistence and adhesion rate were significantly different from those in the hypoxia group and HGF group (P <0. 01) , and the expression of the ICAM-1 protein increased significantly (P <0. 01). Conclusion HGF could inhibit the hy-poxic damage of HPMECs by decreasing the persistence and the adhesive capacity of these cells and inducing the expression of ICAM-1.关键词
缺血性肺动脉高压/肝细胞生长因子/人肺微血管内皮细胞/细胞间黏附分子-1Key words
hypoxic pulmonary hypertension/ hepatocyte growth factor/ human pulmonary microvascular endothelial cells/ intercellular adhesion molecule-1分类
医药卫生引用本文复制引用
郭娜,郭英华,苏龙翔,王雅娟,刘岩,姜学革,刘长庭..肝细胞生长因子对缺氧损伤的肺微血管内皮细胞的保护作用[J].中国医学科学院学报,2013,35(1):1-5,5.基金项目
国家自然科学基金(81000018)、解放军总医院苗圃基金重点项目(10KMZ04)、航天医学基础与应用国家重点实验室开放基金(SMFA11K02)和武器预研重点基金(9140A26040312JB10078) （81000018）
