中国医学科学院学报2013，Vol.35Issue(1)：19-23,5.DOI:10.3881/j.issn.1000-503X.2013.01.004
不同质量浓度磁性c-erbB-2反义探针对SK-Br-3肿瘤细胞株形态及表达的影响
Effects of Magnetic c-erbB-2 Antisense Probe of Different Concentrations on Morphology and Expression of SK-Br-3 Cancer Cell Lines in vitro
摘要
Abstract
Objective To explore the effects of the magnetic c-erbB-2 antisense probe of different concentrations on the morphology and expression of SK-Br-3 cancer cells in vitro. Methods Breast cancer SK-Br-3 cells were transfected for 24 h by antisense probe at an iron concentration of 5, 10, 25, 50, and 100 mg/L, respectively. The distribution and content of iron particles in SK-Br-3 cells was determined by Prussian blue staining, electron microscopy, and atomic absorption spectrometry. Cell viability was observed by trypan-blue exclusion and CCK-8 test. The protein expression of c-erbB-2 was assessed by the Western blot analysis. The changes of the signal strength were considered by magnetic resonance imaging ( MRI). Results c-erbB-2 anti-sense probe was uptake by SK-Br-3 cells in a concentration-dependence manner within a certain range (5 , 10,25 mg/L). When the probe concentration was 25 mg/L, iron content in cells was (18. 38 ±0. 28) pg, the cell vitality, survival, and c-erbB-2 protein expression were reduced significantly (all P < 0. 05) , and the T2 value was lower significantly (P <0. 05). However, the results of 50 mg/L or 100 mg/L group showed no significant difference with the 25 mg/L group (P > 0. 05). Conclusion The magnetic c-erbB-2 antisense probe can effectively transfect and specifically inhibit the expression of SK-Br-3 cell lines at the iron concentration of 25 mg/L.关键词
反义寡脱氧核苷酸/超顺磁性氧化铁/分子探针/SK-Br-3细胞Key words
antisense oligodeoxy-nucleotide/ superparamagnetic iron oxide/ molecular probe/ SK-Br-3 cells分类
医药卫生引用本文复制引用
刘海燕,温志鹏,文明,贺海容,谭书德,李少林..不同质量浓度磁性c-erbB-2反义探针对SK-Br-3肿瘤细胞株形态及表达的影响[J].中国医学科学院学报,2013,35(1):19-23,5.基金项目
国家自然科学基金(30940021)、重庆市自然科学基金(2008BB5209)和重庆市卫生局医学科研基金(062025) （30940021）
