中国医学科学院学报2013，Vol.35Issue(1)：64-68,5.DOI:10.3881/j.issn.1000-503X.2013.01.012
甲状腺乳头状癌并BRAFV600E突变及原癌基因重排蛋白表达与其侵袭性的关系
Invasive Properties of Papillary Thyroid Cancer with Concurrent BRAFV600E Mutation and Rearranged during Transfection Proto-oncogene Protein Expression
摘要
Abstract
Objective To investigate the aggressive properties of papillary thyroid cancer ( PTC) with concurrent BRAFV600Emutation and rearranged during transfection (RET) proto-oncogene protein expression. Methods Fifty pathologically confirmed PTC patients who had received thyroidectomy were enrolled in this study. BRAFV600E mutation was detected by real time polymerase chain reaction ( RT-PCR) , while RET protein expression was measured by immunohistochemical SP method. Clinical and pathological features were compared between the concurrent BRAF mutation and RET protein expression group ( n = 24 ) and BRAFV600E mutation or RET protein expression alone group ( n = 19). Seven patients were ruled out from the final analysis due to the absence of either BRAFV600E mutation or RET protein expression. Results Of these 50 patients, BRAFV600E mutation and RET protein expression were detected in 38 patients (76% ) and 28 patients (56%), respectively. Concurrent BRAFV600E mutation and RET expression was detected in 24 patients (48% ). Compared with the concurrent BRAFv600E mutation and RET protein expression group, the BRAFV600E mutation or RET protein expression alone group had relatively poorer tissue differentiation and higher prognostic score (P = 0. 011, P = 0. 022). Conclusion PTC patients with concurrent BRAFV600E mutation and RET expression present poorer differentiation, more highly aggressive variant in carcinoma tissues, and higher cancer-related mortality risk.关键词
BRAFV600E突变/原癌基因重排蛋白表达/甲状腺乳头状癌/侵袭性Key words
BRAFV600E mutation/ tyrosine kinase receptor protein expression/ papillary thyroid cancer/ aggressiveness分类
医药卫生引用本文复制引用
孟超,高洁,梁军,梁智勇,林岩松..甲状腺乳头状癌并BRAFV600E突变及原癌基因重排蛋白表达与其侵袭性的关系[J].中国医学科学院学报,2013,35(1):64-68,5.基金项目
国家自然科学基金(30970850)和卫生行业科研专项项目(201202012) （30970850）
