浙江大学学报（理学版）2013，Vol.40Issue(2)：216-220,5.DOI:10.3785/j.issn.1008-9497.2013.02.020
天目铁木愈伤组织和芽苗诱导技术
Technique of callus induction and bud seedling of Ostrya rehderiana Chun
摘要
Abstract
The tender stem tips of Ostrya rehderiana Chun was used as explant and its suitable medium compositions were screened for callus induction and shoots redifferentiation through uniform design experiments. The results show that the most suitable culture mediums were as follows; 1/2DR+TDZ 2. 30 mg ? L-1+2,4-D 0. 55 mg ? L-1 for callus induction at base of tender stem tip, the rate of induction was 93. 5% : 1/2DR+TDZ 3. 30 mg ? L-1 +KT 0. 70 mg ? L -1 for shoots redifferentiation, the rate of differentiation was more than 99. 8%. Callus induction and shoots redifferentiation in vitro system of Ostrya rehderiana Chun have been successfully established, and regenera tion buds seedlings and wild plant have the same chromosome number.关键词
天目铁木/愈伤组织/诱导/再分化/均匀设计Key words
Ostrya rehderiana Chun/callus/induction/redifferentiation/uniform design分类
农业科技引用本文复制引用
顾地周,陆爽,巴春影,李媛媛..天目铁木愈伤组织和芽苗诱导技术[J].浙江大学学报（理学版）,2013,40(2):216-220,5.基金项目
国家科技部国家科技攻关计划引导项目(2005BA741C) （2005BA741C）
吉林省科技厅基金资助项目(200705C05). （200705C05）
