安徽中医学院学报2013，Vol.32Issue(2)：57-63,7.DOI:10.3969/j.issn.1000-2219.2013.02.018
千金藤碱抑制非小细胞肺癌A549细胞生长和转移以及对血红素加氧酶-1和表皮生长因子受体基因表达的影响
Inhibitory Effects of Cepharanthine on Growth and Metastasis of Non-Small Cell Lung Cancer A549 Cells and Gene Expression of Heme Oxygenase-1 and Epidermal Growth Factor Receptor in A549 Cells
摘要
Abstract
Objective To investigate the inhibitory effects of cepharanthine (CEP) on the growth, migration, and invasion of human lung cancer cell line A549 and the gene expression of heme oxygenase-1 (HO-1) and epidermal growth factor receptor (EGFR) in A549 cells. Methods The viability of A549 cells treated with CEP was determined by methyl thiazolyl tetrazolium assay, and the morphological changes of A549 cells at different points of treatment with CEP were observed under an inverted microscope. The migration and invasion of A549 cells were evaluated using Transwell chambers and Matrigel-coated Transwell chambers, respectively. The mRNA and protein expression levels of HO-1 and EGFR in A549 cells were measured by RT-PCR and western blot, respectively. Results CEP significantly inhibited the growth of A549 cells, and the cell viability decreased significantly as the concentration of CEP increased and the treatment proceeded. After being treated with 10 g/ml CEP for 24 h, A549 cells became smaller, shrunken, and rounded and showed chromatin condensation. There were significantly fewer cells that crossed the Transwell membrane and Matrigel in the A549 cells treated with CEP (>5. 0 g/ml) than in controls (P< 0. 05). CEP (10. 0 g/ml) significantly decreased the mRNA and protein expression levels of HO-1 and EGFR in A549 cells (P<0. 05). Conclusion CEP can inhibit the growth, migration, and invasion of A549 cells and downregulate the mRNA and protein expression of HO-1 and EGFR in A549 cells. The effects of CEP display certain time and concentration dependence.关键词
千金藤碱/非小细胞肺癌/A549细胞/血红素加氧酶-1/表皮生长因子受体Key words
cepharanthine/ non-small cell lung cancer/ A549 cell/ heme oxygenase-1/ epidermal growth factor receptor分类
医药卫生引用本文复制引用
马克龙,汪远金,周会,李璐,张腾跃,罗晓莹..千金藤碱抑制非小细胞肺癌A549细胞生长和转移以及对血红素加氧酶-1和表皮生长因子受体基因表达的影响[J].安徽中医学院学报,2013,32(2):57-63,7.基金项目
国家自然科学基金项目(81201576) （81201576）
上海市肿瘤研究所青年学者资助项目(SB0908) （SB0908）
安徽省高等学校省级自然科学研究项目(KJ2013Z184) （KJ2013Z184）
安徽中医学院自然科学研究基金项目(2012zr008) （2012zr008）
