安徽中医学院学报2013，Vol.32Issue(2)：73-75,3.DOI:10.3969/j.issn.1000-2219.2013.02.022
高效液相色谱法测定桔梗中木犀草素与芹菜素的含量
Content Determination of Luteolin and Apigenin in Platycodon Grandiflorus by High-performance Liquid Chromatography
摘要
Abstract
Objective To establish a method for determining the content of luteolin and apigenin in Platy-codon Grandiflorus by high-performance liquid chromatography (HPLC). Methods HPLC was performed on an Alltima C18 column (4. 6 mm × 250 mm, 5 μm) with a mobile phase of acetonitrile-0. 5% phosphoric acid (35 : 65) at a flow rate of 1. 0 ml/min, a detection wavelength of 340 nm, and a column temperature of 25 ℃. Results Luteolin and apigenin had a good linear relationship between concentration and peak area in the concentration ranges of 11. 20-67. 20 μg/ml (r=0. 999 6) and 12. 25-73. 50 μg/ml (r=0. 999 9) , respectively. The average recovery rates of luteolin and apigenin were 99. 03% (RSD = 0. 77% , n=6) and 99. 71% (RSD=1. 38%, n= 6), respectively. Conclusion This HPLC method can be used for determining the content of luteolin and apigenin in Platycodon Grandiflorus due to its simplicity, accuracy, and reliability.关键词
桔梗/木犀草素/芹菜素Key words
Platycodon Grandiflorus / luteolin/ apigenin分类
医药卫生引用本文复制引用
周秀娟,储晓琴,桂双英,王举涛,彭代银,金传山..高效液相色谱法测定桔梗中木犀草素与芹菜素的含量[J].安徽中医学院学报,2013,32(2):73-75,3.基金项目
国家十二五科技支撑计划项目(2011BAI04B06) （2011BAI04B06）
安徽省科技攻关项目(12010402084) （12010402084）
安徽省高校省级自然科学研究基金重点项目(KJ2011A194) （KJ2011A194）
安徽省科技计划项目(1206C0805005) （1206C0805005）
