北京中医药大学学报2013，Vol.36Issue(3)：161-165,后插1-后插2,7.DOI:10.3969/j.issn.1006-2157.2013.03.004
健脾益肾化瘀通络方对糖尿病肾病大鼠氧化应激的影响
Influence of Jianpi Yishen Huayu Tongluo Fang on oxidative stress in rats with diabetic nephropathy
摘要
Abstract
Objective To observe the kidney protection effect and influence of Jianpi Yishen Huayu Tongluo Fang on oxidative stress in rats with diabetic nephropathy (DN) induced by streptozocin (STZ). Methods Male Wistar rats (n =74) were intraperitoneally injected STZ (45 mg/kg) for establishing model, and then divided, after successful modeling and according to body weight, into normal grouop, model group, irbesartan (17. 5 mg/kg) group and low-dose (30. 8 mg/kg) and high-dose (123. 2 mg/kg) Jianpi Yishen Huayu Tongluo Fang groups (low-dose group and high-dose group). The body weight and 24-hour urine protein quantitate were measured every 4 weeks. On the 14th week the rats were executed for recording the ration of kidney weight to body weight ( KW/BW) and detecting the levels of serum triglyceride (TG ) , blood urea nitrogen (BUN ) , serum creatinine (Scr ), nitric oxide ( NO ) , malondialdehyde (MDA) and superoxide dismutase (SOD). The pathological changes and scores of kidney tissue were observed and analyzed after staining of HE, Mallory and PASM-HE. Results In low-dose group and high-dose group, the body weight increased significantly (P < 0. 05 ), KW/BW decreased significantly, pathological changes of kidney tissue were relieved, levels of serum TG, BUN and Scr decreased significantly (P < 0. 05 ), activity of serum SOD increased (P < 0. 05 ) and MDA decreased (P < 0. 05) compared with model group. Conclusion Jianpi Yishen Huayu Tongluo Fang can play a role of protecting kidney through antagonizing oxidative stress in DN rats.关键词
健脾益肾化瘀通络方/糖尿病肾病/氧化应激/大鼠Key words
Jianpi Yishen Huayu Tongluo Fang/ diabetic nephropathy/ oxidative stress/ rats分类
医药卫生引用本文复制引用
赵宗江,王颖超,杜磊,张新雪,乌格敦其其格..健脾益肾化瘀通络方对糖尿病肾病大鼠氧化应激的影响[J].北京中医药大学学报,2013,36(3):161-165,后插1-后插2,7.基金项目
国家自然科学基金项目(No.30973831),北京市中医管理局"51510"资助项目(No.JJ2007-006) （No.30973831）
